The Aβ1-42 used in these experiments was acquired from Life Technologies (USA); β-asarone was purchased from NIFDC (Beijing, China), and the purity value is 96.8%; donepezil was obtained from the First Affiliated Hospital of Jinan University (Guangzhou, China); A3 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); CSA was purchased from Selleck (Houston, Texas, USA); 3MA, high-glucose DMEM, FBS, trypsin, and PBS were obtained from Gibco (Gaithersburg, MD, USA); Cell Counting Kit-8 (CCK-8), Cytotoxicity LDH Assay Kit—WST (LDH), and Cellular Senescence Detection Kit—SPiDER-βGal were obtained from Dojindo Molecular Technologies, Inc. (Tokyo, Japan); the bicinchoninic acid (BCA) protein assay kit, Immunol Fluorescence Staining Kit, and Immunohistochemistry Staining Kit were obtained from Beyotime (Shanghai, China); anti-SQSTM1/P62, anti-BECN, anti-LC3 II, anti-beta amyloid, anti-BACE1, anti-synapsin1, anti-Parkin, anti-Pink1, anti-APPL, and anti-PS1 were obtained from Abcam (Cambridge, UK).
Donepezil
Manufactured by Santa Cruz Biotechnology
Sourced in China
Donepezil is a lab equipment product designed for scientific research and investigation. It serves as a key component in various experimental processes, enabling researchers to conduct their studies effectively. The core function of Donepezil is to facilitate specific research-oriented tasks, though its exact intended use may vary depending on the context of the research project.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (2 mentions)
High glucose dmem, by Thermo Fisher Scientific (2 mentions)
Aβ1 42, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Anti pink1, by Abcam (1 mentions)
Anti parkin, by Abcam (1 mentions)
Anti sqstm1 p62, by Abcam (1 mentions)
Anti lc3ii, by Abcam (1 mentions)
Immunol fluorescence staining kit, by Beyotime (1 mentions)
Anti synapsin 1, by Abcam (1 mentions)
Anti ps1, by Abcam (1 mentions)
Anti beta amyloid, by Abcam (1 mentions)
Anti bace1, by Abcam (1 mentions)
Bicinchoninic acid bca protein assay kit, by Beyotime (1 mentions)
Rosiglitazone, by Santa Cruz Biotechnology (1 mentions)
Ketotifen, by Merck Group (1 mentions)
Genistein, by Merck Group (1 mentions)
Crizotinib, by Selleck Chemicals (1 mentions)
Charcoal stripped fetal bovine serum, by Merck Group (1 mentions)
4 protocols using donepezil
1
Alzheimer's Disease Pathogenesis Model
2
Anticancer Compound Screening Protocol
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Fulvestrant and sorafenib were purchased from AK Scientific (Mountain
View, CA); crizotinib from Selleck Chemicals (Houston, TX); rosmarinic
acid, donepezil, and rosiglitazone from Santa Cruz Biotechnology (Dallas,
TX); ketotifen, verapamil, genistein, and GLP-1R agonist from Sigma-Aldrich
(St. Louis, MO); benzoquinoline from Spectrum Chemicals (New Brunswick,
NJ); AFP 07 from Caymen Chemical (Ann Arbor, MI); phenol red from
Amresco (Solon, OH); tetrahydroberberine from Vitas-M (Moscow, Russia).
Ultrapure polysorbate 80 (UP 80) was purchased from NOF Corporation
(White Plains, NY). Dulbecco’s phosphate buffered saline (DPBS)
and RPMI 1640 cell culture media were purchased from Multicell Technologies
(Woonsocket, RI). Charcoal-stripped Fetal bovine serum (FBS) was purchased
from Sigma-Aldrich (St. Louis, MO). Cell lines MDA-MB-231 (HTB-26),
MCF-7 (HTB-22), SK-BR-3 (HTB-30), and BT-474 (HTB-20) were purchased
from ATCC (Manassas, VI). MTS cell proliferation assay was purchased
from Promega (Madison, WI). Duke Standards NIST Traceable Polymer
Microspheres were purchased from Thermo Scientific. FluoSpheres fluorescent
nanoparticles were purchased form Life Technologies (Burlington, ON).
All other chemicals and reagents were purchased from Sigma-Aldrich
(St. Louis, MO) or TCI America (Portland, OR).
View, CA); crizotinib from Selleck Chemicals (Houston, TX); rosmarinic
acid, donepezil, and rosiglitazone from Santa Cruz Biotechnology (Dallas,
TX); ketotifen, verapamil, genistein, and GLP-1R agonist from Sigma-Aldrich
(St. Louis, MO); benzoquinoline from Spectrum Chemicals (New Brunswick,
NJ); AFP 07 from Caymen Chemical (Ann Arbor, MI); phenol red from
Amresco (Solon, OH); tetrahydroberberine from Vitas-M (Moscow, Russia).
Ultrapure polysorbate 80 (UP 80) was purchased from NOF Corporation
(White Plains, NY). Dulbecco’s phosphate buffered saline (DPBS)
and RPMI 1640 cell culture media were purchased from Multicell Technologies
(Woonsocket, RI). Charcoal-stripped Fetal bovine serum (FBS) was purchased
from Sigma-Aldrich (St. Louis, MO). Cell lines MDA-MB-231 (HTB-26),
MCF-7 (HTB-22), SK-BR-3 (HTB-30), and BT-474 (HTB-20) were purchased
from ATCC (Manassas, VI). MTS cell proliferation assay was purchased
from Promega (Madison, WI). Duke Standards NIST Traceable Polymer
Microspheres were purchased from Thermo Scientific. FluoSpheres fluorescent
nanoparticles were purchased form Life Technologies (Burlington, ON).
All other chemicals and reagents were purchased from Sigma-Aldrich
(St. Louis, MO) or TCI America (Portland, OR).
3
Screening for Acetylcholinesterase Inhibitors and Telomerase Activity
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Solvents such as n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc), ethanol (EtOH), and methanol (MeOH), were obtained from the El-Nasr Company for Pharmaceuticals and Chemicals, Egypt. Acetonitrile, methanol (HPLC grade) and DMSO were purchased from (Sigma-Aldrich, St. Louis, MO, USA). Acetyl-cholinesterase inhibitor screening kits (cat. # IACE-100) were purchased from BioAssay Systems (Hayward, CA, USA). Donepezil (Cat # sc-218265) was purchased from Santa Cruz Biotechnology (Texas, USA). TERT ELISA kit (cat. # K4187-100) was purchased from Biovision incorporated (Milpitas, CA, USA), The curcumin standard for the TERT assay was obtained from Sigma-Aldrich, Germany.
4
Alzheimer's Disease Intervention Protocol
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The Aβ 1-42 used in these experiments was acquired from Life Technologies (USA); β-asarone was purchased from NIFDC (Beijing, China,112018-201601), and the purity value is 96.8%; icarrin donepezil was purchased from NIFDC (Beijing, China,110737-201516), and the purity value is 94.2%; donepezil was obtained from the First A liated Hospital of Jinan University (Guangzhou, China); A3 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); CSA was purchased from Selleck (Houston, Texas, USA). 3MA, high-glucose DMEM, FBS, trypsin, and PBS were obtained from Gibco (Gaithersburg, MD, The experimental mice were provided by the Institute of Model Animal Science, Nanjing University, certi cate number: SCXK (SU 2010 0001, B6C3 TG APPSWE PSEN1DE9 85DBO /J strain, SPF grade, 10, half male and half female, body weight (20±2) g 3 months old; C57BL/6 mice, SPF grade, 10, half male and half female, body weight (20±2 g) 3 months old. PC12 (highly differentiated) rat adrenal pheochromocytoma cells (highly differentiated purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Table No. TCR 9 (highly differentiated).
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