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Anti nedd8

Manufactured by Abcam
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Anti-NEDD8 is a primary antibody that recognizes the NEDD8 protein. NEDD8 is a small ubiquitin-like modifier that plays a role in regulating the activity of cullin-RING E3 ubiquitin ligases. The Anti-NEDD8 antibody can be used for the detection of NEDD8 in various applications.

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Lab products found in correlation

Anti cullin 1, by Abcam (2 mentions) Anti myc tag, by Cell Signaling Technology (2 mentions) Anti skp2 antibodies, by Thermo Fisher Scientific (2 mentions) Quantitative reverse transcription polymerase chain reaction rt pcr kit, by Bio-Rad (2 mentions) Reverse transcription system kit, by Promega (2 mentions) Mg132, by Cayman Chemical (2 mentions) Mln4924, by Cayman Chemical (2 mentions) Anti lc3b 2, by Cell Signaling Technology (1 mentions) Anti fbw7, by Abcam (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Anti p27kip1 antibody, by BD (1 mentions) Anti cdh1, by Cell Signaling Technology (1 mentions) 20s proteasome assay kit, by Cayman Chemical (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions) Anti p27kip1, by BD (1 mentions) Bortezomib, by Cayman Chemical (1 mentions) Anti flag, by Merck Group (1 mentions) Anti actin, by Santa Cruz Biotechnology (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

NEDD8 (7 citations) Anti-NEDD8 (ab81264) (2 citations)

3 protocols using anti nedd8

1

Gartanin-Mediated Protein Degradation

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Gartanin was obtained from Chromadex with 99% purity (Irvine, CA). MG-132 and MLN4924 were obtained from Cayman Chemical Inc. (Ann Arbor, MI). Antibodies against Ubc12, ubiquitin, and β-tubulin were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-Skp2 antibodies were purchased from Invitrogen (Grand Island, NY). Anti-Myc-tag, anti-LC3B-II, anti-FBXW2, anti-Cdh1, and anti-β-TrPC antibodies were from Cell Signaling Inc. (Boston, MA). Anti-Cullin-1, anti-FBW7 and anti-NEDD8 antibodies were from Abcam (Cambridge, MA). Anti-p27/Kip1 antibody was from BD Biosciences (Billerica, MA). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Sigma. Reverse Transcription System kit and was from Promega (Mandison, WI). A quantitative reverse transcription polymerase chain reaction (RT-PCR) kit was from Bio-Rad (Hercules, CA).
Pham V., Rendon R., Le V.X., Tippin M., Fu D.J., Le T.H., Miller M., Agredano E., Cedano J, & Zi X. (2019). Gartanin is a novel NEDDylation inhibitor for induction of Skp2 degradation, FBXW2 expression and autophagy. Molecular carcinogenesis, 59(2), 193-201.
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2

Proteasomal Regulation by FKB in Cancer

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FKB with 99% purity was isolated from kava extracts by LKT Laboratories, Inc. (St. Paul, MN). Bortezomib, MG-132 and MLN4924 were obtained from Cayman Chemical Inc. (Ann Arbor, MI). Antibodies against Ubc12, ubiquitin, and β-tubulin were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-Skp2 antibodies were purchased from Invitrogen (Grand Island, NY). Anti-Myc-tag and Anti-cleaved-PARP antibodies were from Cell Signaling (Boston, MA). Anti-Cullin-1 and anti-NEDD8 antibodies were from Abcam (Cambridge, MA). Anti-p27/Kip1 and p21/WAF1 antibodies were from BD Biosciences (Billerica, MA). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Sigma. The Reverse Transcription System kit and was from Promega (Mandison, WI). A quantitative reverse transcription polymerase chain reaction (RT-PCR) kit was from Bio-Rad (Hercules, CA). Ubiquitylation Assay Kit and 20S Proteasome Assay Kit were from Cayman and Abcam, respectively.
Li X., Pham V., Tippin M., Fu D., Rendon R., Song L., Uchio E., Hoang B.H, & Zi X. (2019). Flavokawain B targets protein neddylation for enhancing the anti-prostate cancer effect of Bortezomib via Skp2 degradation. Cell Communication and Signaling : CCS, 17, 25.
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3

Proteomic Analysis of Ubiquitin-Mediated Pathways

Cited 2 times
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The following antibodies were used: anti-CDC53, anti-Sic1, anti-CDC4, anti-Actin, anti-HA (Santa Cruz); anti-Flag (Sigma); anti-ubiquitin (Dako), anti-NEDD8 (Abcam), anti-Rpn11 [95] (link), anti-Rpn8 [96] , anti-Rpn2 [72] (link).
Bramasole L., Sinha A., Gurevich S., Radzinski M., Klein Y., Panat N., Gefen E., Rinaldi T., Jimenez-Morales D., Johnson J., Krogan N.J., Reis N., Reichmann D., Glickman M.H, & Pick E. (2018). Proteasome lid bridges mitochondrial stress with Cdc53/Cullin1 NEDDylation status. Redox Biology, 20, 533-543.
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