Samples were quantified using the PowerQuant® System (Promega) on the 7500 RealTime PCR System (Applied Biosystems) and amplified using PowerPlex® Fusion 6C (Promega) kit with 1.0 ng template DNA input in a final reaction volume of 25 µl. Applied Biosystem® Veriti 96-Well Thermal Cycler (ThermoFisher) with a 29 PCR cycles program was used for the amplification. Negative and positive controls were run with the samples. Capillary electrophoresis was performed on the Applied Biosysetm® 3500xL Genetic Analyzer (ThermoFisher) with an injection time of 24 s and a voltage of 1.2 kV.
Applied biosystem veriti 96 well thermal cycler
Manufactured by Thermo Fisher Scientific
The Applied Biosystems® Veriti 96-Well Thermal Cycler is a laboratory instrument designed for performing polymerase chain reaction (PCR) experiments. It features a 96-well sample block that can precisely control the temperature of samples during the thermal cycling process required for DNA amplification.
Automatically generated - may contain errors
Lab products found in correlation
Powerquant system, by Promega (2 mentions)
7500 real time pcr system, by Thermo Fisher Scientific (2 mentions)
Powerplex fusion 6c, by Promega (1 mentions)
Applied biosystems 3500xl genetic analyzer, by Thermo Fisher Scientific (1 mentions)
Yfiler plus pcr amplification kit, by Thermo Fisher Scientific (1 mentions)
Genemapper id x software v1, by Thermo Fisher Scientific (1 mentions)
Powerplex fusion 6c system, by Promega (1 mentions)
Chelex 100, by Bio-Rad (1 mentions)
3 protocols using applied biosystem veriti 96 well thermal cycler
1
Forensic DNA Typing Workflow
2
Y-STR Multiplex Amplification and Profiling
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Multiplex amplification of Y-STRs was performed on an Applied Biosystem® Veriti™ 96‐well thermal cycler (ThermoFisher Scientific) by using the Yfiler™ Plus PCR Amplification Kit (ThermoFisher Scientific) according to the manufacturer's protocol utilising 1 ng of genomic DNA. The amplified DNAs were then electrophoresed on the 24-capillary Applied Biosystems® 3500xL Genetic Analyzer (ThermoFisher Scientific) and the fragment analysis was performed using GeneMapper® ID-X software v.1.4 (ThermoFisher Scientific).
3
DNA Extraction and Profiling Protocol
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DNA extraction was performed according to Chelex® 100 (Bio-Rad Laboratories) protocol, using 200 µl of 5% Chelex solution (also elution volume). No prior incubation with water was made [19, 20] . Negative extraction controls were processed with the samples. The amount of DNA in each sample was determined using PowerQuant® System (Promega) on the 7500 RealTime PCR System (Applied Biosystems) following manufacturer's instructions. The extracted DNA was amplified using PowerPlex® Fusion 6C System (Promega). 1.0 ng of template DNA was used for PCR amplification in a total reaction volume of 25 µl. 29 PCR cycles were used for amplifying the samples on Applied Bio-system® Veriti 96-Well Thermal Cycler (ThermoFisher). Negative and positive amplification controls were run together with the samples. Amplification products were separated and detected by capillary electrophoresis on the Applied Biosystem® 3500xL Genetic Analyzer (ThermoFisher). An injection time of 24 s and a voltage of 1.2 kV were applied.
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