Mycoplasmincin

The human pancreatic cancer cell lines AsPC-1, MIA PaCa-2, PANC-1, HuP-T4, HuP-T3, PSN1, and CFPAC-1 were purchased from American Type Culture Collection (ATCC) and provided by Suzhou Truway Biotechnology Inc. All cell genetic information was analyzed and downloaded from the Cancer Cell Line Encyclopedia (CCLE) or Catalogu of Somatic Mutations in Cancer (COSMIC) database. Cells were cultured in RPMI 1640, McCoy's 5a, MEM or DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin under the recommended conditions. The ATCC has performed morphological, cytogenetic and DNA profile analyses for characterization of these cell lines. The cell passages were limited to 15 generations for all experiments in this study. Mycoplasma contamination was excluded using the antibiotic mycoplasmincin (InvivoGen) and was periodically examined using a MycoFluor Mycoplasma Detection Kit (Invitrogen, #M7006).

SUP-B15 and K562 cell lines were purchased from ATCC and cultured in RPMI 1640 (Basal Media, China) supplemented with 10% fetal bovine serum (FBS, Moregate, Batch No. 827106). Cell line identities were validated by using short tandem repeat profiling analysis according to the American National Standard ANS-0002-2011 at the laboratory of VivaCell Bioscience Co. The cell passages were limited to 15 generations for all experiments in this study. Mycoplasma contamination was excluded using the antibiotics Mycoplasmincin (InvivoGen) and periodically examined using MycoFluor Mycoplasma Detection Kit (Invitrogen, #M7006).