Worms were imaged by mounting on 5% agarose pads in 3 mM levamisole. Images of reporter worms were acquired using a Zeiss Axiovert 200 microscope, a Hamamatsu Orca 100 cooled CCD camera and Zeiss axiovision software. Images of HSF-1::GFP were captured using a Leica SP5 II laser scanning confocal microscope equipped with a 100X/1.4 N.A. oil immersion lens and HyD detectors. Acquisition parameters were kept identical across samples. For clarity, images were cropped and brightness/contrast was adjusted across the entire image using Adobe Photoshop.
Axiovert 200
Manufactured by Hamamatsu Photonics
The Axiovert 200 is an inverted research microscope designed for a variety of applications. It features a stable, vibration-resistant stand and provides high-quality optical performance. The microscope can be equipped with a range of accessories to support various imaging techniques.
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Lab products found in correlation
3 protocols using axiovert 200
1
Imaging Worms and HSF-1 Expression
2
Worm Imaging for Polyglutamine Aggregates
3
Fluorescence Microscopy Quantification of Yeast Cells
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Fluorescence microscopy of living yeast cells was performed as described (Jungbluth et al., 2010 (link)) with a Zeiss Axiovert 200 equipped with a Hamamatsu camera, EGFP and rhodamine filter sets, and a 63x Plan Apochromat oil lens (NA 1.4). Transmission light and fluorescence images were collected in a single plane using the image acquisition software Volocity 5.03 (Perkin Elmer, Waltham, MA). The software Fiji was used for image processing and fluorescence quantification (Schindelin et al., 2012 (link)). Flow cytometer measurements were performed as described by Hasenjäger et al. (2019) (link). Briefly, yeast cells in logarithmic growth phase were treated with sodium azide (10 mM final concentration) and transferred to a 96-well plate. The measurements were performed with an Attune NxT (ThermoFisher) equipped with an autosampler. Mean red fluorescence of six independent measurements was used to generate the graph; error bars shows the SEM.
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