2 14c acetic acid

AMIO hydrochloride (reference A8423), ALLO (A8003), DPEN (P4875), 5FU (F6627), INDI (Y0000788), INDO (I7378), METHI (M8506), METHO hydrate (M8407), NIF (N7634), RIF (R3501), SUL (S8139), (+)-etomoxir sodium salt hydrate (E1905), and thapsigargin (T9033) were purchased from Sigma Aldrich (Saint-Quentin-Fallavier, France). Dimethyl sulfoxide (DMSO), digitonin, oleic acid, stearic acid, palmitic acid, palmitoyl-CoA, palmitoyl-l-carnitine, octanoyl-l-carnitine, malate (disodium salt), glutamate (monosodium salt), acetic acid, insulin, l-carnitine, fatty acid-free bovine serum albumin (BSA), and Percoll were also bought from Sigma Aldrich. William’s E medium, Dulbecco’s phosphate-buffered saline (PBS) Gibco™, glutamine, penicillin, streptomycin, formaldehyde, Nile red, and Hoechst 33342 dyes were obtained from Thermo Fischer Scientific (Waltham, MA). TRO (A11981) and lomitapide (A12778) were purchased from Adooq BioScience (Irvine, CA). Tunicamycin (10-2111) and tauroursodeoxycholic acid (TUDCA; 10-2782) were purchased from Focus Biomolecules (Plymouth Meeting, PA). Radiolabeled [U-¹⁴C]palmitic acid and [2-¹⁴C]acetic acid, and sodium salt were purchased from PerkinElmer (Waltham, MA). Hydrocortisone hemisuccinate was obtained from SERB Laboratories (Paris, France). Fetal bovine serum (FBS) was purchased from Eurobio (Les Ulis, France) and GE Healthcare (Little Chalfont, UK).

Doxycycline, palmitic acid, TOFA, c75, MTT (3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide), and anti-GAPDH antibody were purchased from Sigma (Sigma-Aldrich, St. Louis, MO). FASN (C20G5) and acetyl-CoA carboxylase (C83B10) antibodies were purchased from Cell Signaling (Cell Signaling Technology, Inc., Danvers, MA, USA). Anti-rabbit and anti-mouse IgG, and HRP-linked antibodies were from GE (GE Healthcare Life Science, Pittsburgh, PA, USA). BRG1 antiserum [70 (link)] was used in western blot and ChIP experiments. The BRM antibody (ab15597) used in western blots was purchased from Abcam (Cambridge, MA, USA). D-¹⁴C-Glucose, ³H-Leucine, ³⁵S-Methionine and [2–¹⁴C] acetic acid were purchased from PerkinElmer (PerkinElmer Life Sciences, Waltham, MA, USA). ADAADi was prepared as described [28 (link)].