Anti ikk β antibody

Manufactured by Abcam

Anti-IKK-β antibody is a primary antibody that targets and binds to the IKK-β protein. IKK-β is a serine/threonine protein kinase that plays a crucial role in the activation of the NF-κB signaling pathway.

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Lab products found in correlation

2 protocols using anti ikk β antibody

Biochemical and Inflammatory Markers in Cellular Assays

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The serum biochemical reagents included ALT (LOT: 140121005), AST (LOT: 140221004), ALP (LOT: 140321002), TC (LOT: 141621013), TG (LOT: 141721003), LDL (142021004), and HDL (LOT. 142121006). TNF-a (E-EL-R2856-96T), IL-6 (E-EL-R0015-96T), and IL-1β (E-EL-R0012-96T) ELISA kits were purchased from Elabscience Biotechnology Co., Ltd. Anti-Caspase-8 antibody (Abcam, ab108333), anti-Caspase-3 antibody (Abcam, ab184787), anti-Cytochrome C antibody (Abcam, ab133504), and anti-IKK-β antibody (Abcam, ab124957) were purchased from Abcam. BCL2 monoclonal antibody (60178-1-1 g) and GAPDH (60004-1-Ig) antibody were obtained from Proteintech Group, Inc. NF-κB p65 antibody, phospho-NF-κB p65 antibody (LOT:17), a-SMA (CST, #19245), IκB-α antibody (CST, #4812), and phospho-IκB-α antibody (CST, #2859) were provided by Cell Signaling Technology, Inc. Chemiluminescence ECL Detection Kit (P90719) was obtained from Bobst Biotechnology.

Zhi G., Shao B., Zheng T., Mu J., Li J., Feng Y., Zhu S., Dang Y., Liu F, & Wang D. (2023). Exploring the molecular mechanism of Gan Shuang granules for the treatment of non-alcoholic steatohepatitis using network pharmacology, molecular docking, and experimental verification. Frontiers in Pharmacology, 14, 1082451.

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Inflammatory Modulation by GC and LPS

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GC (PubChem CID: 161120) and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (Merck KGaA). TNF-α, IL-1β, IL-6 ELISA kits and anti-IKK-β antibody were purchased from Abcam. Anti-CD40, anti-ICAM-1, anti-VCAM-1, anti-iNOS, anti-NF-κB p65, anti-phosphorylated (p)-IκB-α, anti-IκB-α, anti-β-actin, anti-histone and goat anti-mouse IgG-HRP antibodies were purchased from Santa Cruz Biotechnology, Inc. Phenylmethanesulfonyl fluoride and RIPA lysis buffer were purchased from Abcam. PVDF membranes, the BCA protein concentration assay kit and the SDS-PAGE gel preparation kit were obtained from Beyotime Institute of Biotechnology. The ECL plus kit was purchased from Cusabio Technology LLC.

Zhang R., Guo N., Yan G., Wang Q., Gao T., Zhang B, & Hou N. (2021). Ginkgolide C attenuates lipopolysaccharide-induced acute lung injury by inhibiting inflammation via regulating the CD40/NF-κB signaling pathway. International Journal of Molecular Medicine, 47(4), 62.

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