Ampicillin ap

The bacterial strains and plasmids used are listed in S1 Table. Escherichia coli strains DH5α and expression strain BL21DE3pLysS as well as the B. pseudomallei strain K96243 were cultured in Luria-Bertani (LB) medium or LB agar at 37°C. Unless stated otherwise, the concentrations of antibiotics added to LB medium for E. coli were as follows: ampicillin (Ap, Sigma-Aldrich, Germany), 100 μg/ml and/or chloramphenicol (Cm, Sigma-Aldrich, Germany), 25 μg/ml.

All bacterial strains and plasmids used are listed in Table S1. Escherichia coli strains DH5α and expression strains BL21(DE3)pLysS as well as the B. pseudomallei strain K96243 were cultured in lysogeny broth (LB) medium, LB agar or M9 minimal medium at 37°C. The concentrations of antibiotics added were as follows, unless stated otherwise: 100 μg/ml ampicillin (Ap, Sigma-Aldrich, Germany), 50 µg/ml kanamycin (Km, Carl Roth, Germany) and 25 μg/ml chloramphenicol (Cm, Sigma-Aldrich, Germany).

Bacterial strains, bacteriophages and plasmids used in this study are listed in Table 3. The E. coli O157:H7 C7927 bacterial strain was used for the cultivation of phage ΦV1032 (link). Homologous recombination was carried out in a lysogenic strain E. coli O157:H7 C7927 (ΦV10) bearing the lambda Red expression plasmid pKD46. For the construction of ΦV10 reporter phage, bacterial strains were grown in Luria-Bertani (LB) broth (Difco Laboratories, MI) or LB agar plates supplemented with antibiotics as needed [ampicillin (Ap), 100 μg ml⁻¹; kanamycin (Kan), 50 μg ml⁻¹ (Sigma-Aldrich, MO)]. Salt-Optimized Carbon broth medium (SOC) (Clontech Laboratories, Inc, CA) was used for recovery of cells after electroporation. Modified tryptone soya broth (Oxoid Ltd., UK) containing 1% casamino acids (VWR International, PA) with novobiocin (Sigma-Aldrich, MO) of 8 mg l⁻¹ (mTSB + n) was used for ground beef enrichment as specified by the USDA-FSIS protocol33 34 . Cell dilutions were done in phosphate buffered saline (PBS) (8 mM Na₂HPO₄, 6 mM NaH₂PO_4, 145 mM NaCl, pH7.6). Phage buffer (50 mM Tris, 100 mM MgCl₂, pH7.6) was used to dilute and preserve the phage stock. LB Top agar (1% (wt/vol) tryptone (Becton Dickinson, NJ), 1% (wt/vol) NaCl (Macron, PA), 0.5% (wt/vol) yeast extract (Hardy Diagnostics, CA) and 0.6% (wt/vol) agar (Alfa Aesar, MA)) was used for the overlay plaque assay.