Cells grown on coverslips were fixed for 10 min in 2% (w/v) sucrose and 2% (v/v) paraformaldehyde at room temperature followed by PBS washes. Coverslips were blocked in 0.2% (w/v) fish gelatin and 0.5% (w/v) BSA in PBS. Cells were incubated with primary antibodies and after PBS washes, cells were incubated with appropriate Alexa fluor secondary antibodies followed by washes in PBS + 0.1% Triton. IF-FISH was carried out using a TelC-Cy3 PNA telomere probe (PNA Bio). DNA was stained with DAPI, and digital images captured using NIS-Elements BR (Nikon) with a Nikon Eclipse 80i microscope and an Andor CCD camera.
Telc cy3 pna telomere probe
Manufactured by PNA Bio
The TelC-Cy3 PNA telomere probe is a DNA-mimicking molecule that binds specifically to telomeric DNA sequences. It is labeled with the fluorescent dye Cy3, allowing for the visualization and quantification of telomere length in cells.
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Lab products found in correlation
2 protocols using telc cy3 pna telomere probe
1
Immunofluorescence and Telomere FISH
2
Immunofluorescence and Telomere FISH
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