To prepare the Ca2+-free medium, Hank’s balanced salt solution (HBSS; 14175-095, Gibco) was supplemented with 20 mM hydroxyethylpiperazine ethane sulfonic acid (HEPES; Dojindo), 0.5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA; E3889, Sigma), 1 mM magnesium chloride (MgCl2; M8266, Sigma), and 1 mM magnesium sulfate (MgSO4; 746452, Sigma) (Kim et al., 2015 (link)). To prepare the Ca2+ medium, HBSS was supplemented with 20 mM HEPES, 1 mM MgSO4, and 1 mM calcium chloride (CaCl2; 18230S0301, JUNSEI). The Ca2+ medium did not include EGTA, a calcium chelator, or MgCl2, a supplemented ion. To represent normal conditions, CO2-independent culture medium (18045088, Gibco) containing 0.5% (v/v) FBS, 4 mM l -glutamine (LS002-1, Welgene), 100 U/ml penicillin, and 100 μg/ml streptomycin was used. Yoda1 (SML1558), methyl-β-cyclodextrin (MβCD; 332615), ML-7 (I2764), gadolinium (Ⅲ) chloride hexahydrate (G7535), and thapsigargin (T9033) were purchased from Sigma-Aldrich. Nocodazole (S2775) was purchased from Selleckchem. Cytochalasin D (ab143484) was obtained from Abcam.
Cacl2
Manufactured by Junsei
Sourced in Japan
Calcium chloride (CaCl2) is a chemical compound that is commonly used as a desiccant and in various industrial applications. It is a colorless, crystalline solid that is highly soluble in water. CaCl2 is known for its ability to absorb moisture from the air, making it useful in applications where the removal of water is required.
Automatically generated - may contain errors
Lab products found in correlation
Thapsigargin t9033, by Merck Group (1 mentions)
Hepes, by Dojindo Laboratories (1 mentions)
Cytochalasin d ab143484, by Abcam (1 mentions)
Mgso4, by Junsei (1 mentions)
M8266, by Merck Group (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Mgso4, by Merck Group (1 mentions)
2 protocols using cacl2
1
Ca2+ Homeostasis Modulation Techniques
2
CsA Release from CsA-Algi Gel
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A cumulative release profile of CsA from CsA-Algi gel was measured using a dialysis method. A total of 10.868 μg of CsA was loaded into PLGA microspheres, which were then mixed with alginate solution at a concentration of 10 wt%, then cross-linked using CaCl 2 (Junsei, Tokyo, Japan) .
The prepared gel was put inside a cellulose dialysis bag (MW cutoff 3500; Thermo Fischer Scientific, Rockford, IL, USA) with 3 ml of 0.1 M phosphate buffer (pH 7.4) containing 0.01% (w/v) Tween 80. This method avoids the loss of microspheres during the experiment. The dialysis bag with the gel was suspended in the phosphate buffer (16), then incubated at 37°C. At predetermined intervals, 1 ml of suspension was collected, and the same amount of fresh medium was added. The drug release was quantified using HPLC.
The prepared gel was put inside a cellulose dialysis bag (MW cutoff 3500; Thermo Fischer Scientific, Rockford, IL, USA) with 3 ml of 0.1 M phosphate buffer (pH 7.4) containing 0.01% (w/v) Tween 80. This method avoids the loss of microspheres during the experiment. The dialysis bag with the gel was suspended in the phosphate buffer (16), then incubated at 37°C. At predetermined intervals, 1 ml of suspension was collected, and the same amount of fresh medium was added. The drug release was quantified using HPLC.
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