PA signals of four CNPs concentrations (5, 10, 15 and 20 mg/mL) in 2% agarose gel phantom were firstly measured to investigate the relationship between concentrations and PA signals using the VEVO LASR PA imaging system (Vevo®LAZR, Visual Sonics, Inc., Canada), and the saline groups were used as control. The PA signals of both groups were quantitatively analyzed. In addition, the laser energy of our PA system (<30mJ) cannot lead to phase transition of CNPs after multiple tests previously, therefore, Laser Diode Controller (ADR-1085, Mid-river, China) and color Doppler ultrasound MyLab 90 (Esaote, Italy) were used for contrast-enhanced ultrasound imaging (CEUS). Briefly, after exposure to the laser (808 nm, 1 W/cm2) for 10 s, four CNPs concentrations (5, 10, 15, 20 mg/mL) in 2% agarose gel phantom were observed with B-mode and contrast-mode, and the saline groups were also used as control. After ultrasonic images acquisition, the images of ROI (region of interest) were quantitatively analyzed with “average gray scale” using DFY ultrasound imaging analysis software (invented by the Institution of Ultrasound Imaging of Chongqing Medical University, Chongqing, China).
Vevo lasr pa imaging system vevo lazr
Manufactured by Fujifilm
Sourced in Canada
The VEVO LASR PA imaging system is a high-performance photoacoustic imaging platform designed for preclinical research. The system uses laser-induced photoacoustic signals to generate detailed images of biological structures and functions.
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Lab products found in correlation
2 protocols using vevo lasr pa imaging system vevo lazr
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Quantifying Contrast-Enhanced PA and US Imaging
2
Photothermal Characterization of DOC-CNP
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The PA signals
of four DOC-CNP solution concentrations (5, 10,
15, and 20 mg/mL) in 2% agarose gel phantom were first measured to
investigate the relationship between the concentrations and the PA
signal using the VEVO LASR PA imaging system (VevoLAZR, Visual Sonics,
Inc., Canada). The PA images were collected, and the PA signals were
quantitatively analyzed.
Similarly, the DOC-CNP solutions of
the four concentrations (5, 10, 15, and 20 mg/mL) were continuously
irradiated with a laser (808 nm, 2.0 W/cm2) until 50 °C,
and the temperature was monitored every 3 s with an infrared thermal
imager (FOTRIC, USA).
The DOC-CNP solution (20 mg/mL) was introduced
into a 24-well plate
and then irradiated with a laser (808 nm, 2.0 W/cm2). The
temperature of the DOC-CNP solution was monitored by an infrared thermal
imager. When the sample temperature reached 50 °C, the laser
was shut off. While the sample was restored to room temperature, the
laser irradiation was recovered, and the process was repeated three
times. The temperature was recorded every 3 s.
of four DOC-CNP solution concentrations (5, 10,
15, and 20 mg/mL) in 2% agarose gel phantom were first measured to
investigate the relationship between the concentrations and the PA
signal using the VEVO LASR PA imaging system (VevoLAZR, Visual Sonics,
Inc., Canada). The PA images were collected, and the PA signals were
quantitatively analyzed.
Similarly, the DOC-CNP solutions of
the four concentrations (5, 10, 15, and 20 mg/mL) were continuously
irradiated with a laser (808 nm, 2.0 W/cm2) until 50 °C,
and the temperature was monitored every 3 s with an infrared thermal
imager (FOTRIC, USA).
The DOC-CNP solution (20 mg/mL) was introduced
into a 24-well plate
and then irradiated with a laser (808 nm, 2.0 W/cm2). The
temperature of the DOC-CNP solution was monitored by an infrared thermal
imager. When the sample temperature reached 50 °C, the laser
was shut off. While the sample was restored to room temperature, the
laser irradiation was recovered, and the process was repeated three
times. The temperature was recorded every 3 s.
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