D xylose solutions

Xylose concentration was determined by HPLC. Samples were injected into an Ultimate 3000 HPLC (Thermo Fisher Scientific Inc., Waltham, MA, USA) equipped with an Aminex-HPX-87H (Bio-Rad Laboratories Inc., Hercules, California, USA). Separation occurred at 40°C with H₂SO₄ solution (5 mM) at a flow rate of 0.6 ml/min. The standard curve was constructed using D(+)xylose solutions (Merck KGaA, Darmstadt, Germany). A refractive index detector, Shodex RI-101 (Shodex, Munich, Germany), was applied for peak detection.

Xylose concentration was determined by liquid chromatography (HPLC) as previously described [24 (link)]. Briefly, 10 μL of each sample appropriately diluted were injected into a Ultimate 3000 HPLC (Dionex, Thermo Fisher Scientific Inc., Waltham, MA, USA) equipped with a Aminex HPX-87H Column (Bio-Rad Laboratories, Inc. Hercules, California, USA). For detection, a Refractive Index Detector Shodex RI-101 (Shodex, Munich, Germany) was used. Separation occurred at 45 °C with a H₂SO₄ solution (5 mM) at a flow rate of 0.6 mL/min. The standard curve was constructed using d(+)xylose solutions (Merck KGaA, Darmstadt, Germany) containing up to 3 g/L.