The 10-day-old ICR mice were i.g. inoculated with 105 TCID50 CVA6 strains. At 1 dpi, 3 dpi, 5 dpi, and 7 dpi, mock- and CVA6-infected mice (n = 7–10) were euthanized. The brain and spleen cells were extracted and staining as described previously [31 (link),32 ]. The antibodies used in FACS analysis were purchased from Biolegend Inc., CA, USA: APC-conjugated CD3 antibody (#100312), AF700-conjugated B220 antibody (#103210), BV711-conjugated CD4 antibody (#100549), Biotin anti-CD8 antibody (#100704), FITC-conjugated CD44 antibody (#103006), PE-conjugated CD69 antibody (#104507), FITC-conjugated CD25 antibody (#101907), FITC-conjugated Ly-6c antibody (#128006), PE-conjugated CD62L antibody (#104407), PE-conjugated CD11c antibody (#117308), PerCP/Cy5.5-conjugated Ly-6G antibody (#127615), APC-conjugated F4/80 antibody (#123115), APC/Cy7-conjugated CD45 antibody (#103116), PerCP/Cy5.5-conjugated IgM antibody (#406512), PE/Cyanine7 Streptavidin (#405206), anti-CD16/32 antibody (#101330). LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Thermo Fisher Scientific Co., Ltd, Waltham, USA) was used to distinguish dead cells from living ones. Flow cytometry measurement was performed using the BD LSRFortessa FACS Canto and the data was analyzed by the FlowJoX software.
Apc conjugated cd3 antibody
Manufactured by BioLegend
Sourced in United States
The APC-conjugated CD3 antibody is a laboratory reagent used in flow cytometry applications. It binds to the CD3 surface antigen expressed on T cells, allowing their identification and quantification. The antibody is conjugated to the fluorescent dye Allophycocyanin (APC), which can be detected using appropriate flow cytometry instrumentation.
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Lab products found in correlation
Apc conjugated f4 80 antibody, by BioLegend (1 mentions)
Fitc conjugated cd44 antibody, by BioLegend (1 mentions)
Pe conjugated cd69 antibody, by BioLegend (1 mentions)
Pe cyanine7 streptavidin, by BioLegend (1 mentions)
Pe conjugated cd8 antibody, by BioLegend (1 mentions)
Fitc conjugated cd4 antibody, by BioLegend (1 mentions)
2 protocols using apc conjugated cd3 antibody
1
Immune Response to CVA6 Infection in Mice
2
Multicolor Flow Cytometry of Mouse Immune Cells
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For flow cytometry analysis of the mouse tissue samples, single-cell suspensions were prepared and stained for flow cytometry with the following antibodies: APC-conjugated CD3 antibody (BioLegend, 100236); FITC-conjugated CD4 antibody (BioLegend, 100510); PerCP-conjugated CD45 antibody (BioLegend, 103130); PE-conjugated CD8 antibody (BioLegend, 100708). After 15 minutes incubation at room temperature, cells were washed three times for 10 minutes each time with PBS, the cells were then resuspended in PBS and analyzed by flow cytometry. Data were analyzed with FlowJo.
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