Cy5-conjugated anti-CD3, fluorescein isothiocyanate (FITC)–conjugated anti-CD8, Cy5-conjugated anti-CD8, Cy5-conjugated anti-CD4, phycoerythrin (PE)–conjugated anti-CD158b (killer cell immunoglobulin-like receptor 2DS2 [KIR-2DS2]), PE-conjugated anti-NKB1 (KIR-3DL1), and FITC-conjugated anti-CD28 were obtained from BD PharMingen. PE-conjugated anti-CD158d (KIR-2DL4) was obtained from R&D Systems and PE-conjugated anti-CD158a/h (KIR-2DL1/2DS2) and PE-conjugated anti-CD158b1/b2 (KIR-2DL2/2DL3/2DS2) were obtained from Beckman Coulter Immunotech. Five microliters of each PE-conjugated anti-KIR antibody was mixed to form a cocktail and used to stain the cells (19 (link)). All labeling procedures were performed on ice in PBS containing 10% horse serum and normal human AB serum (Invitrogen) and sodium azide. Cell staining was performed as previously described (20 (link)), and the stained cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences).
Cy5 conjugated anti cd8
Manufactured by BD
Sourced in United States
Cy5-conjugated anti-CD8 is a fluorescently labeled monoclonal antibody that specifically binds to the CD8 surface antigen. It is designed for use in flow cytometry and other immunoassay applications.
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2 protocols using cy5 conjugated anti cd8
1
Multicolor Flow Cytometry Immunophenotyping
2
Thymocyte Isolation and Flow Cytometry
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At the indicated time points after the various treatments of mice, thymocytes were isolated, washed twice, and resuspended in ice-cold PBS before staining either with phycoerythrin (PE)-labeled anti-CD4 and Cy5-conjugated anti-CD8 (Pharmingen, San Diego, CA, USA) antibodies or with fluorescein isothiocyanate (FITC)-labeled annexin V in binding buffer. Cell-bound fluorescence was analyzed in a blinded fashion using a FACSCalibur (Beckton Dickinson).
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