Spectra acquisition was performed on MicroFlex LT mass spectrometer (Bruker Daltonik, Bremen, Germany) using MALDI Biotyper software version 3.1. Bruker Bacterial Test Standard was used for a calibration.
Microflex lt mass
Manufactured by Bruker
Sourced in Germany
The MicroFlex LT is a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer designed for rapid, accurate, and sensitive analysis of a wide range of samples. The instrument provides high-performance mass analysis for applications such as protein identification, peptide mapping, and small molecule characterization.
Automatically generated - may contain errors
Lab products found in correlation
Bacterial test standard, by Bruker (1 mentions)
Mbt compass library db 4110, by Bruker (1 mentions)
Mbt compass ivd software, by Bruker (1 mentions)
Vitek 2 instrument, by bioMérieux (1 mentions)
Spectramax i3 multi mode microplate reader, by Molecular Devices (1 mentions)
Columbia blood agar plates, by bioMérieux (1 mentions)
Wpa co8000 cell density meter, by Harvard Bioscience (1 mentions)
4 protocols using microflex lt mass
1
MALDI-TOF Bacterial Identification Protocol
2
MALDI-TOF MS for Microbial Identification
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Species identification of isolates was performed with MALDI-TOF MS in the clinical laboratory Unilabs at Skaraborg Hospital on a MicroFlex LT mass spectrometer (Bruker Daltonics, Germany) with BioTyper software v2.0 using default parameter settings as part of the routine clinical practice as described elsewhere (Ljungstrom et al., 2015 (link); Enroth et al., 2019 (link)). Spectral scores above 2.0 were used as cut-off for correct identification. At the time of the study, the Bruker microorganism database MBT Compass Library DB-4110 (Bruker Daltonics, Germany) released in April 2011 was used.
3
Bacterial Identification and Antibiotic Resistance
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Bacterial identification was routinely performed with MALDI-TOF mass spectrometry with Microflex LT mass (Bruker, Mannheim, Germany) using the MBT Compass IVD software (Bruker Daltonics, Bremen, Germany) according to manufacturer’s instructions. Antimicrobial susceptibility testing (AST) was performed with the VITEK 2 instrument (BioMerieux, Durham, NC, USA) for the following antibiotics: ampicillin, piperacillin/tazobactam, cefuroxime, ceftazidime, cefotaxime, cefepime, meropenem, ertapenem, amikacin, gentamicin, tobramycin, ciprofloxacin, trimethoprim/sulfamethoxazole, nitrofurantoin and the Kirby–Bauer disk diffusion method for trimethoprim, cefixime, and fosfomycin. The results were interpreted in accordance with EUCAST guidelines according to the version applicable in a given year. Based on the pattern of antibiotic resistance, we classified the isolates as susceptible to all tested antibiotics (S), non-susceptible to at least one agent in three or more antimicrobial categories (MDR), or non-susceptible to at least one agent in all antimicrobial categories except two or less (XDR).
4
Isolation and Characterization of Clinical Staphylococcus epidermidis
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Clinical S. epidermidis isolates (Table 1 ) were isolated by sonication from the pacemaker as described before42 (link) and characterized at the Institute of Medical Microbiology of the University of Zurich. For each clinical sample colonies with different morphology were archived if present, otherwise only one colony was frozen. Species identification was done by Microflex LT mass spectrometer (Bruker Daltonik) as described before43 (link).
The strains were stored in Lennox Broth (LB) supplemented with 20% glycerol at −80 °C. Bacteria were grown on solid agar plates containing Tryptic Soy Broth (TSB, BD) or columbia blood agar plates (BioMerieux) for ~40 h, if not indicated otherwise. Overnight cultures (o/n) were inoculated from fresh plates in 5 ml of TSB in 50 -ml conical tubes and incubated for 18–20 h under shaking conditions (220 rpm) at 37 °C. Bacterial growth was measured by optical density at 600 nm (OD600) using a WPA CO8000 Cell Density Meter (Biochrom, Berlin, Germany) or a SpectraMax i3 multi-mode microplate reader (Molecular Devices, San José, CA, USA).
The strains were stored in Lennox Broth (LB) supplemented with 20% glycerol at −80 °C. Bacteria were grown on solid agar plates containing Tryptic Soy Broth (TSB, BD) or columbia blood agar plates (BioMerieux) for ~40 h, if not indicated otherwise. Overnight cultures (o/n) were inoculated from fresh plates in 5 ml of TSB in 50 -ml conical tubes and incubated for 18–20 h under shaking conditions (220 rpm) at 37 °C. Bacterial growth was measured by optical density at 600 nm (OD600) using a WPA CO8000 Cell Density Meter (Biochrom, Berlin, Germany) or a SpectraMax i3 multi-mode microplate reader (Molecular Devices, San José, CA, USA).
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