Morphine d3

Manufactured by Merck Group

Sourced in United States, Switzerland

Morphine-d3 is a stable isotope-labeled standard for the analysis of morphine. It serves as an internal standard for the quantification of morphine in various analytical applications, such as bioanalysis and pharmaceutical analysis.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (8 mentions) Morphine, by Merck Group (6 mentions) Codeine, by Merck Group (5 mentions) Codeine d3, by Merck Group (4 mentions) Milli q system, by Merck Group (3 mentions) Thebaine, by Merck Group (2 mentions) Tramadol, by Merck Group (1 mentions) Phenylbutazone, by Merck Group (1 mentions) Buprenorphine d4, by Merck Group (1 mentions) Ketoprofen d3, by Merck Group (1 mentions) Optima grade acetonitrile acn, by Thermo Fisher Scientific (1 mentions) Optima grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Papaverine, by Merck Group (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Isopropanol, by Thermo Fisher Scientific (1 mentions) Noscapine, by Merck Group (1 mentions) Xylene substitute, by Avantor (1 mentions) Absolute ethanol, by Avantor (1 mentions) 6 acetylmorphine, by Merck Group (1 mentions)

Spelling variants of this product

Morphine (95 citations)

9 protocols using morphine d3

Quantification of Veterinary Pharmaceutical Substances

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Morphine, Morphine-d₃, tramadol, ¹³C-tramadol-d₃, buprenorphine-d₄, chlorpromazine-d₃, and phenylbutazone were obtained as solutions from Sigma-Aldrich Chemie GmbH (Buchs, Switzerland). Butorphanol and buprenorphine were obtained as solutions from ReseaChem GmbH (Burgdorf, Switzerland) and Lipomed AG (Arlesheim, Switzerland), respectively. Ketoprofen-d₃ and phenylbutazone-(diphenyl¹³C₁₂) were purchased as powders Sigma-Aldrich Chemie GmbH (Buchs, Switzerland). Detomidine, acepromazine, meloxicam, ketoprofen, chlorpromazine, flunixin and fluphenazine were analytical standards available as powders in the authors’ laboratory. All solvents and other chemicals were purchased from Sigma-Aldrich Chemie GmbH (Buchs, Switzerland). All standards were dissolved in methanol, except for meloxicam that was dissolved in dimethylformamide, to yield stocks of 1 mg/ml. Firocoxib was obtained as an injectable for veterinary use. Chromatography-grade water used for LC-MS/MS analysis was processed by a PURELAB Option-Q system by ELGA LabWater (Labtec Services AG, Villmergen, Switzerland).

Madry M.M., Spycher B.S., Kupper J., Fuerst A., Baumgartner M.R., Kraemer T, & Naegeli H. (2016). Long-term monitoring of opioid, sedative and anti-inflammatory drugs in horse hair using a selective and sensitive LC-MS/MS procedure. BMC Veterinary Research, 12, 84.

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HPLC-MS Analysis of Noscapine and Morphine

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Optima-grade acetonitrile (ACN) and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Deionized (DI) water (18.2 MΩ cm at 25 °C) was obtained from a Milli-Q system (Millipore-Sigma, Burlington, MA, USA). Noscapine standard (European Pharmacopeia reference standard) and morphine-D₃ (100 μg mL⁻¹ in methanol; certified reference material) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Shetge S.A, & Redan B.W. (2022). Assessment of Dry Heating, Water Rinsing, and Baking on Concentrations of the Opium Alkaloid Noscapine in Poppy Seeds. ACS food science & technology, 2(3), 541-547.

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Quantitative Analysis of Opioid Alkaloids

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Optima-grade acetonitrile and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Deionized (DI) water (18.2 MΩ cm⁻¹ at 25 °C) was obtained from a Milli-Q system (Millipore-Sigma, Burlington, MA, USA). Standards of morphine (1 mg mL⁻¹ in MeOH), codeine (1 mg mL⁻¹ in MeOH), thebaine (1 mg mL⁻¹ in MeOH), morphine-D₃ (100 μg mL⁻¹ in MeOH), codeine-D₃ (100 μg mL⁻¹ in MeOH), and morphine N-oxide (100 μg mL⁻¹ in MeOH), were all purchased from Sigma-Aldrich (St. Louis, MO, USA).

Shetge S.A., Dzakovich M.P., Cooperstone J.L., Kleinmeier D, & Redan B.W. (2020). Concentrations of the Opium Alkaloids Morphine, Codeine, and Thebaine in Poppy Seeds are Reduced after Thermal and Washing Treatments but are Not Affected when Incorporated in a Model Baked Product. Journal of agricultural and food chemistry, 68(18), 5241-5248.

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Quantification of Opiate Alkaloids in Poppy Seeds

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Representative images of poppy seeds and the chemical structures of five OAs are shown in Figure 1 and Figure 2. Morphine, codeine, thebaine, noscapine, papaverine, Morphine-d_3, and codeine-d₃ were purchased from Sigma (St. Louis, MO, USA). The reference compounds were more than 99% pure, with identity and purity confirmed by chromatographic and spectral data (HRMS). HPLC-grade solvents such as acetonitrile, methanol, isopropanol, and formic acid were procured from Fisher Scientific, Waltham, MA, USA. Ultrapure water (18.2 MΩ.cm) was purified using the Milli-Q system (Millipore, Bedford, MA, USA).

Avula B., Katragunta K., Adams S.J., Wang Y.H., Chittiboyina A.G, & Khan I.A. (2023). Applicability of LC-QToF and Microscopical Tools in Combating the Sophisticated, Economically Motivated Adulteration of Poppy Seeds. Foods, 12(7), 1510.

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Quantification of Morphine and Metabolites

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All the standard molecules used in the study were purchased from Sigma-Aldrich (St. Louis, MO, USA): morphine, morphine-3-glucuronide, morphine-6-glucoronide solutions (1.0 mg/mL in methanol each standard) and 6-acetylmorphine (1.0 mg/mL in acetonitrile standard solution). Deuterated internal standards (ISs) were purchased from Sigma-Aldrich as well: morphine-D₃ (1.0 mg/mL in methanol standard solution), 6-acethylmorphine-D₃ (1.0 mg/mL in acetonitrile), morphine-3-glucuronide-D₃ (100 µg/mL in methanol with 0.05% of NaOH) and morphine-6-glucuronide-D₃ (100 µg/mL methanol/water (1:1, v/v)). Solvents used in the extractive processes were purchased from Sigma-Aldrich (methanol, hydrochloric acid, chloroform, dichloromethane and ethyl acetate) and VWR Chemicals (Radnor, PA, USA) (acetone, acetonitrile, isopropanol and n-hexane). Buffer pH 6.88 solution was purchased from PanReac (Barcelona, Spain). For histological inclusions, absolute ethanol and xylene substitute were purchased from VWR Chemicals and high fusion point paraffin pearls Histowax® were purchased from International Medical Products (Brussel, Belgium).

Di Candia D., Boracchi M., Gentile G., Giordano G, & Zoja R. (2022). Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago. Forensic Toxicology, 40(2), 302-311.

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Quantitative Analysis of Opioids

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Morphine, methadone, codeine, 6-MAM, EDDP standards, and Morphine-d3 and methadone-d3 internal standards (IS) were purchased from Sigma Aldrich (Milan, Italy). For the extraction procedure, distilled water (H₂O) for analysis, sodium hydroxide (NaOH), methanol (MeOH) for analysis (≥99.9%), hydrochloric acid for analysis (37%), dichloromethane for analysis stabilized with amylin (≥99.9%), 2-propanol (99.8%) and ammonium hydroxide (32%) of reagent grade were purchased from Carlo Erba (Cornaredo, Italy). For the UHPLC analysis, methanol for UHPLC-MS (99.99%), ultrapure water for UHPLC-MS and formic acid (FA) for LC-MS (≥99%) were purchased from Carlo Erba (Cornaredo, Italy). All reagents and laboratory consumables were stored according to the manufacturer’s instructions.

Buratti E., Mietti G., Cippitelli M., Cerioni A., Froldi R., Cingolani M, & Scendoni R. (2023). Detection of Three Opioids (Morphine, Codeine and Methadone) and Their Metabolites (6-Monoacetylmorphine and 2-Ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine) in Larvae of Lucilia sericata Species by UHPLC-TF-MS and Validation. Molecules, 28(12), 4649.

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Quantification of Opioids in Urine

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Morphine (M-005), Morphine-D3 (M-006), Morphine-D6 (M-086), Codeine (C-006), Codeine-D3 (C-007) and Codeine-D6 (C-041) of 1 mg/ml methanol solution with 99% purity were obtained from Cerilliant (Sigma-Aldrich). Reagents used for the derivation of the analytes and the ISs, Bis (trimethylsilyl) trifluoroacetamide (BSTFA) with 10 g/L trimethylchlorosilane was purchased from Supelco (Bellefonte, PA, USA). Urine samples positive for illicit drugs and used for study analyses were obtained from the Division of Clinical Toxicology, Tri-Service General Hospital. Drug-free urine samples were provided by a member of the research group. All samples were kept at -20°C until analyzed.

Shang H., Tang S., Chuang C., Chen S., & Chuang W. (2020). Optimized procedures for simultaneous quantitation of low concentration levels of morphine and codeine in urine using gas chromatography–mass spectrometry. Journal of Medical Sciences, 40(2), 68-68.

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HPLC Analysis of Opioid Compounds

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Methanol (HPLC grade) was purchased from Merck (Darmstadt, Germany). Ammonium carbonate and formic acid (both analytical grade) were supplied by Acros Organics (Morris Plains, NJ, USA). Acetic acid and ammonium acetate (both analytical grade) were obtained from VWR International (Leuven, Belgium).
Norcodeine, morphine, M3G, morphine-d 3 , codeine-d 3 and M3G-d 3 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Codeine was purchased from Norwegian Medical Depot (Oslo, Norway), and C6G from Lipomed (Arlesheim, Switzerland). M6G was supplied by Chiron (Trondheim, Norway).

Frost J., Løkken T.N., Brede W.R., Hegstad S., Nordrum I.S, & Slørdal L. (2015). A validated method for simultaneous determination of codeine, codeine-6-glucuronide, norcodeine, morphine, morphine-3-glucuronide and morphine-6-glucuronide in post-mortem blood, vitreous fluid, muscle, fat and brain tissue by LC-MS. Journal of analytical toxicology, 39(3).

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Analytical Standards for Pharmaceuticals

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Analytical and isotopically labeled standards were all of high purity grade (>98%). Paracetamol (acetaminophen), metronidazole, codeine, morphine, sulfamethoxazol, ciprofloxacin, carbamazepine, metronidazole-hydroxyl, ibuprofen, piperacillin, diclofenac, mefenamic acidhydroxyl, Paracetamol-d4, morphine-d3, gabapentin-d10 and ibuprofen-d3 were purchased from Sigma-Aldrich (Buchs, Switzerland), sulfamethoxazol-d4 from LGC standards (Wesel, Germany) and gabapentin from Toronto Research Chemicals (Ontario, Canada). HPLC-grade methanol (MeOH) and acetonitrile (ACN), as well as HCl and formic acid were purchased from Sigma-Aldrich (Buchs, Switzerland). Ultrapure water (18.2 MΩ×cm) was obtained using a Millipore system (Millipore Ltd., Bedford, USA).

Daouk S., Fleury-Souverain S, & Daali Y. (2015). Development of an LC-MS/MS Method for the Assessment of Selected Active Pharmaceuticals and Metabolites in Wastewaters of a Swiss University Hospital. Chimia, 69(11).

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