Architect i1000 system

Serum samples were used for antibody detection against several pathogens. Anti-dengue immunoglobulin G (IgG) and IgM were detected using SD BIOLINE Dengue Duo rapid test (Abbott), following manufacturer’s instructions. Anti-Human Immunodeficiency Virus (HIV), Hepatitis B Virus (HBV), and Hepatitis C Virus (HCV) as well as anti-IgM against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-COV-2) were detected by chemiluminescence immunoassay using the Architect I1000 system (Abbott), following manufacturer’s instructions.

In Changhai Hospital, Chemiluminescence microparticle immunoassay (CMIA) was performed using an Architect I 1000 system (Abbott Laboratories, Abbott Park, IL, USA) to determine tacrolimus blood concentration. The lower limit of quantitation (LOD) was 1.5 ng·mL⁻¹ and the calibration range was between 2.0 and 30 ng·mL⁻¹.28 (link) In Huashan Hospital, enzyme multiplied immunoassay technique (EMIT) was used with a SYVA Viva-Emit 2000 kit (Siemens Healthcare Diagnostics Inc., Germany). The calibration range was 2.0–30 ng·mL⁻¹ and LOD was 2 ng·mL⁻¹.29 There were systematic biases between CMIA and EMIT. Thus, to correct these biases, the data from Huashan Hospital were converted to their corresponding equivalents in terms of the reported bioassays using the following formula:28 (link)
CMIA=0.93*EMIT+0.36.

The vancomycin dosing regimen consisted of 500 mg (every 6 h, 8 h, 12 h, 24 h, 48 h) and 1000 mg (every 8 h, 12 h), for 1.5–2 h intravenous infusion. The TDM usually occurred 0.5–2 h before and after the fourth or fifth dose of vancomycin, which were regarded as steady-state concentrations. All the TDM data were collected. All serum concentrations of vancomycin were determined via the chemiluminescence microparticle immunoassay method with the ARCHITECT i1000 system (Abbott Laboratories, Chicago, IL, USA). The coefficient of variation was less than 10%, and the linear range was between 3 and 100 mg/L.