Uas atg7rnai

Manufactured by Bloomington Drosophila Stock Center

The UAS-Atg7RNAi is a genetic construct that allows for the knockdown of the Atg7 gene in Drosophila. Atg7 is a key regulator of autophagy, a cellular process that is important for degradation and recycling of cellular components. The UAS-Atg7RNAi construct can be used to study the role of autophagy in various biological processes in Drosophila.

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Lab products found in correlation

Chloroquine, by Merck Group (1 mentions) Gmr gal4, by Bloomington Drosophila Stock Center (1 mentions) Ptc gal4, by Bloomington Drosophila Stock Center (1 mentions) Cg gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas rheb, by Bloomington Drosophila Stock Center (1 mentions) Uas rhebrnai, by Bloomington Drosophila Stock Center (1 mentions) Uas atg9rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas atg1 rnai, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using uas atg7rnai

Drosophila Autophagy Monitoring Protocols

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Flies were raised at 25 °C following standard procedures. The fly strains used in this study were as follows: GMR-Gal4, ptc-Gal4, Cg-Gal4 (BL7011), UASp-mCherry-GFP-Atg8a (BL37749), UAS-Atg7^RNAi (BL27707), and UAS-Leon^RNAi#1 (BL31886) were obtained from the Bloomington Stock Center; Leon^RNAi#2 (v17567) was obtained from the Vienna Drosophila Resource Center (VDRC); UAS-Atg1, UAS-Flag-Leon, UAS-Flag-ED-Leon, leon¹, leon^19-2 mutants were described previously [29 (link), 58 (link)]; UAS-Bsk-DN and UAS-Puc were gifts from Jui-Chou Hsu (National Tsing Hua University, Taiwan). UAS-Flag-Ubpy was generated by subcloning Ubpy from SD04548 (Drosophila Genomics Resource Center (DGRC) into the pUAST vector. To monitor autophagy in Drosophila larval fat bodies, early third instar larvae fed on normal food or starved in 20% sucrose for 4 h were collected for experiments. The larval fat body cell clones were generated using the FLP-out technique as described previously [59 (link)]. In CQ treatment experiments, second instar larvae were transferred to normal food supplemented with 10 mg/ml chloroquine (Sigma) for 6 h.

Pai Y.L., Lin Y.J., Peng W.H., Huang L.T., Chou H.Y., Wang C.H., Chien C.T, & Chen G.C. (2023). The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy. Cell Death & Disease, 14(8), 540.

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Autophagy regulation in Drosophila

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Flies were raised at 25°C following standard procedures unless otherwise noted. The following Drosophila strains were used: Atg7^d14, Atg7^d77 (Juhász et al., 2007 (link)), UAS-Atg1 (Scott et al., 2007 (link)), Dl-Gal4, Su(H)GBE-Gal4, (Zeng et al., 2010 (link)), How-Gal4^ts (gift from Bruno Lemaitre, EPFL), NP1-Gal4, UAS-TSC1, UAS-TSC2 (Tang et al., 2013 (link)), UAS-Rheb (RRID:BDSC_9688), UAS-S6K^KQ (RRID:BDSC_6911), UAS-Tor^TED (RRID:BDSC_7013), UAS-Atg1^RNAi (RRID:BDSC_44034), UAS-Rheb^RNAi (RRID:BDSC_33966), UAS-Atg7^RNAi (RRID:BDSC_34369), UAS-Atg9^RNAi (RRID:BDSC_34901), UAS-Atg12^RNAi (RRID:BDSC_34675), and Df(2R)Exel7142 (RRID:BDSC_7886) flies were obtained from the Bloomington Stock Center; UAS-Patj^RNAi (RRID:FlyBase_FBst0473750), UAS-Atg13^RNAi (RRID:FlyBase_FBst0475239), UAS-Atg16^RNAi (RRID:FlyBase_FBst0477819), UAS-Atg17^RNAi (RRID:FlyBase_FBst0476692), UAS-Atg18^RNAi (RRID:FlyBase_FBst0477193), UAS-Vps34^RNAi (RRID:FlyBase_FBst0472170), UAS-TSC1^RNAi (RRID:FlyBase_FBst0454493), and UAS-TSC2^RNAi (RRID:FlyBase_FBst0470276) were obtained from the Vienna Drosophila Resource Center.

Wen J.K., Wang Y.T., Chan C.C., Hsieh C.W., Liao H.M., Hung C.C, & Chen G.C. (2017). Atg9 antagonizes TOR signaling to regulate intestinal cell growth and epithelial homeostasis in Drosophila. eLife, 6, e29338.

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