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2 protocols using sdhb ab178423

1

Molecular Markers in Parkinson's Disease

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MPP+ and MPTP were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-TH (F-11, sc-25269), dopamine transporter (DAT, sc-32258), GDH2 (sc-293459), GDH1 (sc-515542), and pyruvate dehydrogenase E1α (PDH-E1α) (sc-377092) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-BDNF (ab108319), SHDA (ab137040), and SDHB (ab178423) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-GFAP (#80788), NR2A (#4205), GluA1 (#13185), Bax (#14796), and GluA2 (#13607) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). EAAT1 (20785-1-AP), EAAT2 (22515-1-AP), Nrf2 (16396-1-AP), Bcl-2 (16396-1-AP), and GAPDH (60004-1) antibodies were purchased from Proteintech Group (Rosemont, IL, USA). DyLight 488 goat anti-mouse IgG (H + L) (70-GAM4882) and DyLight 594 goat anti-rabbit IgG (H + L) (70-GAR5942) were purchased from Multi Sciences (Hangzhou, China). Horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG and HRP-labeled goat anti-mouse IgG were purchased from Beyotime Biotechnology (Shanghai, China). Mito-SOX™ Red Mitochondrial Superoxide Indicator was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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2

Detailed MPTP Neurotoxicity Protocol

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MPTP was purchased from Sigma-Aldrich (St. Louis, MO, USA); anti-TH (F-11, sc-25269) and dopamine transporter (DAT, sc-32258) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA); anti-BDNF (ab108319), SHDA (ab137040), and SDHB (ab178423) antibodies were purchased from Abcam (Cambridge, MA, USA); anti-Bax (#14796), Phospho-PI3 Kinase (#17366), PI3 K (#4249), Phospho-Akt (#4060), and Akt (#4685) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); Nrf2 (16396-1-AP), Bcl-2 (16396-1-AP), and GAPDH (60004–1) antibodies were purchased from the Proteintech Group (Rosemont, IL, USA); DyLight 488 goat anti-mouse IgG (H + L) (70-GAM4882) and DyLight 594 goat anti-rabbit IgG (H + L) (70-GAR5942) were purchased from Multi Sciences (Hangzhou, China); horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG and HRP-labeled goat anti-mouse IgG were purchased from Beyotime Biotechnology (Shanghai, China); and the GSH and GSSG Assay Kit (#S0053) and Cu/Zn-SOD and Mn-SOD Assay Kit with WST-8 (#S0103) were purchased from Beyotime Biotechnology (Shanghai, China).
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