12 bit ccd camera

Morphological changes occurring after application of shikonin were observed with a Nikon inverted microscope (Eclipse TE 300, Nikon). TT cells (2 mL) were seeded into 6-well plates at a density of 2 × 10⁵ cells/mL. After allowing them to adhere overnight, cells were incubated with shikonin (IC₅₀) and cell morphology was observed after 24 and 48 h. Images were taken with a Nikon 12-bit CCD camera (Nikon).

Images of the TG-treated cells and the controls were captured using a Nikon inverted microscope (Eclipse TE 300; Nikon, Tokyo, Japan) and a Nikon 12-bit CCD camera (Nikon). Staining with 4′,6-diamidine-2′-phenylindole dyhydrochloride (DAPI) was performed following 24, 48 and 72 h incubation with the TG or UA. The cells were fixed using methanol and the slides were centrifuged with cell suspensions in FlexiPERM 8-chambers (Heraeus, Hanau, Germany) at 300 × g for 5 min (5×10⁶ cells/chamber). The slides were incubated in a moist chamber at room temperature for 20 min in diluted DAPI stock solution (1 μg/ml calcium and magnesium free-phosphate-buffered saline; CMF-PBS) and washed with CMF-PBS. Images were captured using a Leica DFC 300 FX camera (Leica, Wetzlar, Germany).