Cells were treated with different concentrations of the following drugs: aphidicolin (A0781, Sigma-Aldrich), camptothecin (CPT; C9911, Sigma-Aldrich), cisplatin (146262, Hospira), concanamycin A (27689, Sigma-Aldrich), deoxy-nucleosides (dA D8668, dG 854999, dT T1895, dC D3897, Sigma-Aldrich), hydroxyurea (HU; H-8627, Sigma-Aldrich), rapamycin (R0395, Sigma-Aldrich).
Hydroxyurea hu h 8627
Manufactured by Merck Group
Hydroxyurea (HU; H-8627) is a laboratory reagent used for various research applications. It is a chemical compound that inhibits the enzyme ribonucleotide reductase, which is involved in the synthesis of DNA. This product is commonly used in cell biology, molecular biology, and biochemistry research.
Automatically generated - may contain errors
Lab products found in correlation
Aphidicolin, by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Cisplatin, by Pfizer (1 mentions)
Camptothecin cpt c9911, by Merck Group (1 mentions)
Concanamycin a, by Merck Group (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Axioskop 2 microscope, by Zeiss (1 mentions)
Cellquest software, by BD (1 mentions)
α factor, by GenScript (1 mentions)
3 protocols using hydroxyurea hu h 8627
1
Drug Treatment Effects on Cells
2
Cell Cycle Arrest Induction Protocol
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Cells were grown in YPD to logarithmic phase at 30ºC, treated for 2 h with 3 μM α-factor (RP01002; GenScript, Piscataway, NJ) to arrest cells in G1, 0.2 M hydroxyurea (HU; H8627; Sigma-Aldrich, St. Louis, MO) to arrest cells in S phase, and 20 μg/ml nocodazole (M1404; Sigma-Aldrich) to arrest cells in G2/M. Samples were analyzed by FACS to confirm the cell cycle arrest using a Becton-Dickinson FACSort flow cytometer and Cell Quest software (BD Biosciences, Boston, MA). Cell cycle stages were determined based on nuclear position and cell morphology in propidium iodide–stained cells using the Zeiss Axioskop 2 microscope (Carl Zeiss, Peabody, MA) as described previously (Calvert and Lannigan, 2010 ).
3
Cytarabine and Hydroxyurea Protocol
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Cytosine-β-d -arabinofuranoside (C1768-100MG) was purchased from Sigma and dissolved in RPMI 1640 into a final concentration of 10 mg/mL. Hydroxyurea (HU) (H8627) purchased from Sigma was also used in combination with cytarabine (see supplemental Information ).
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