Pms2 clone mrq 28

Manufactured by Cell Marque

Sourced in United States

PMS2 (clone MRQ-28) is an immunohistochemical antibody used for the detection of PMS2 protein. PMS2 is a DNA mismatch repair protein that is part of the post-replicative DNA mismatch repair system.

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Lab products found in correlation

Mlh1 clone g168 15, by Biocare Medical (1 mentions) Msh6 clone bc 44, by Biocare Medical (1 mentions) Msh2 clone g219 1129, by Roche (1 mentions)

Close spelling variants of this product

MRQ-28

2 protocols using pms2 clone mrq 28

Immunohistochemical Analysis of DNA Mismatch Repair Proteins

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Immunohistochemical analysis of DNA mismatch repair proteins MLH1 and MSH2 was performed on Ventana Benchmark Ultra automaton in 214 tumor samples. To increase the sensitivity of immunohistochemistry, we also studied the expression of MSH6 and PMS2 by the same technique [12 (link), 19 (link)].
Four μm tissue sections from formalin-fixed paraffin-embedded tissue were stained with antibodies against hMLH1 (clone G168-728; Cell marque), hMSH2 (clone G219-1129; Cell Marque), hMSH6 (clone BC/44; Cell Marque), and PMS2 (clone MRQ-28; Cell Marque). Absence of nuclear staining in tumor cells with the presence of positive staining in surrounding cells (lymphocytes or normal glandular cells) was interpreted as a loss of expression of these proteins.

Sekal M., Ameurtesse H., Chbani L., Ouldim K., Bennis S., Abkari M., Boulouz A., Benajah D.A., Benjelloun B., Ousadden A., Ait Taleb K., Ait Laalim S., Toghrai I., Mazaz K., Arifi S., Mellas N., El Rhazi K., Harmouch T., Ibrahimi S.A, & Amarti Riffi A. (2015). Epigenetics could explain some Moroccan population colorectal cancers peculiarities: microsatellite instability pathway exploration. Diagnostic Pathology, 10, 77.

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Immunohistochemical Profiling of MMR Proteins

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Four monoclonal antibodies, which are directed against different mismatch repair (MMR) proteins, were used in the investigation: MLH1 (clone G168-15, 1:50; Biocare, Concorde, CA, USA), MSH2 (clone G219-1129, ready to use; Ventana, Tucson, AZ, USA), MSH6 (clone BC-44; 1:50; Biocare) and PMS2 (clone MRQ-28, 1:50; Cell Marque, Rocklin, CA, USA). For a tumor to be considered MMR-proficient, immunoreactivity for MLH1, MSH2, MSH6, and PMS2 was required, whereas loss of at least one of the four markers characterized MMR-deficient tumors. All MMR-deficient tumors were subsequently tested for microsatellite instability (MSI).

Rössler O., Betge J., Harbaum L., Mrak K., Tschmelitsch J, & Langner C. (2017). Tumor size, tumor location, and antitumor inflammatory response are associated with lymph node size in colorectal cancer patients. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 30(6).

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