Gen5ch 2

Manufactured by Agilent Technologies

The GEN5CH 2.0 is a data analysis and acquisition software designed for Agilent's line of microplate readers. It provides a user-friendly interface for setting up and running experiments, as well as analyzing the resulting data. The software supports a variety of microplate formats and assay types, allowing researchers to efficiently manage their microplate-based experiments.

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Lab products found in correlation

Synergy 4 multi mode microplate reader, by Agilent Technologies (2 mentions) Ifn γ elisa kits, by Neobioscience (1 mentions)

3 protocols using gen5ch 2

Quantification of Soluble CD24 by ELISA

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The serum levels of soluble CD24 (sCD24) in patients and healthy individuals were detected and quantitated by enzyme-linked immunosorbent assay (ELISA). Commercially available human CD24 ELISA kits (Cloud-clone, Wuhan, China) were used according to the manufacturer’s instructions. The results were measured on a Syngery^TM 4 Multi-Mode Microplate Reader with software GEN5CH 2.0 (BioTek, Winooski, VT).

Zheng X., Wang P., Song J., Tang Y., Xie Y., Jin X., Zhu D., Fang X., Wei C., Li R., Hu F, & Li Z. (2023). Soluble CD24 is an inflammatory biomarker in early and seronegative rheumatoid arthritis. Annals of Medicine, 55(2), 2246370.

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Quantifying Soluble SR-A Levels

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Sera from patients or arthritic mice were aliquoted and stored at −80 °C until use. Commercially available human SR-A ELISA kit (Sino Biological Inc., Beijing, China) and mouse SR-A ELISA kit (USCN, Wuhan, China) were used according to the manufacturer’s instructions with modifications. The results were measured on a Synergy^TM 4 Multi-Mode Microplate Reader with software GEN5CH 2.0 (BioTek, Winooski, VT).

Hu F., Jiang X., Guo C., Li Y., Chen S., Zhang W., Du Y., Wang P., Zheng X., Fang X., Li X., Song J., Xie Y., Huang F., Xue J., Bai M., Jia Y., Liu X., Ren L., Zhang X., Guo J., Pan H., Su Y., Yi H., Ye H., Zuo D., Li J., Wu H., Wang Y., Li R., Liu L., Wang X.Y, & Li Z. (2020). Scavenger receptor-A is a biomarker and effector of rheumatoid arthritis: A large-scale multicenter study. Nature Communications, 11, 1911.

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Cytokine Detection in Supernatants

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The cell culture supernatants or serum were collected and stored at -80°C until use.
Commercial available mouse/human IL-17A and IFN-γ ELISA kits (NEOBIOSCIENCE, Beijing, China) were used to detect the cytokines according to the manufacturer's instructions. The results were measured on the Synergy TM 4 Multi-Mode Microplate Reader with software GEN5CH 2.0 (BioTek, Winooski, VT).

Wang P., Song J., Bai M., Zheng X., Xie Y., Tang Y., Li X., Fang X., Yuan J., Ren L., Ye H., Su Y., Wang S., Li Z., & Hu F. (2021). Natural killer-like B cells promote Th17 cell differentiation and exacerbate rheumatoid arthritis.

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