Docosahexaenoic acid (DHA) was obtained from Cayman Chemical (Ann Harbor, MI), RPMI and DMEM media and fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA), and mouse interleukin 4 (IL-4) from Sigma Chemical (St Louis, MO, USA). Coelenterazine was purchased from MedChem Express (Monmouth Junction, NJ). Mouse diets were purchased from DYETS, Inc. (Bethlehem, PA). For the ω-6 diet, 30% of energy (134 g/kg) was provided by corn oil, and the ω-6 to ω-3 ratio was 18:1. For the ω-3 diet 30% of energy was provided by menhaden oil (134 g/kg) and the ω-6 to ω-3 ratio was 1:8 as previously described [5 (link)].
Rpmi and dmem media
Manufactured by Thermo Fisher Scientific
Sourced in United States
RPMI and DMEM media are widely used cell culture media formulations developed by Roswell Park Memorial Institute (RPMI) and Dulbecco's Modified Eagle's Medium (DMEM), respectively. These media are designed to support the growth and maintenance of a variety of cell types in vitro. They provide the necessary nutrients, vitamins, and other components required for cell proliferation and survival.
Automatically generated - may contain errors
4 protocols using rpmi and dmem media
1
Dietary Omega-3 and Omega-6 Fatty Acids
2
Dietary Omega-3 and Omega-6 Fatty Acids
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Docosahexaenoic acid (DHA) was obtained from Cayman Chemical (Ann Harbor, MI, USA), RPMI and DMEM media and fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA, USA), and mouse interleukin 4 (IL-4) from Sigma Chemical (St Louis, MO, USA). Mouse diets were purchased from DYETS, Inc. (Bethlehem, PA). For the ω−6 diet, 30% of energy (134g/kg) was provided by corn oil, and the ω−6 to ω−3 ratio was 18:1. For the ω−3 diet 30% of energy was provided by menhaden oil (134g/kg) and the ω−6 to ω−3 ratio was 1:8 as previously described [11 (link)].
3
Dietary Omega-3 and Omega-6 Fatty Acids
4
Evaluation of DHA Effects in Cells
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Docosahexaenoic acid (DHA, ω-3 FAs) was obtained from Cayman Chemical (Ann Harbor, MI, USA). RPMI and DMEM media and fetal bovine serum were purchased from Invitrogen (Carlsbad, CA, USA); mouse IL-4 from Sigma Chemical (St Louis, MO, USA).
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