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Spirit transmission microscope

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The Spirit transmission microscope is a laboratory instrument used to magnify and observe small-scale specimens. It utilizes a system of lenses and light to produce enlarged images of samples, allowing for detailed examination and analysis.

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Lab products found in correlation

Diamond knife, by Electron Microscopy Sciences (2 mentions) Osmium tetraoxide, by Agar Scientific (2 mentions) Formvar coated copper grid, by Electron Microscopy Sciences (2 mentions) Uranyl acetate, by Agar Scientific (2 mentions) Orius sc200b camera, by Ametek (2 mentions) Durcupan resin, by Merck Group (2 mentions) Uranyl acetate, by Electron Microscopy Sciences (2 mentions)

2 protocols using spirit transmission microscope

1

Ultrastructural Analysis of Spinal Cord Lesions

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Animals were perfused with 3% glutaraldehyde, 2% paraformaldehyde, 0.1M phosphate buffer (pH 7.4), 0.7% (w/v) NaCl and spinal cords were dissected and post-fixed overnight. Specimen were post-fixed in 1% osmium tetraoxide (Agar Scientific, Stansted, UK), dehydrated, stained en bloc with uranyl acetate (Agar Scientific, Stansted, UK), and embedded in Durcupan resin (Sigma-Aldrich, UK). Ultrathin sections (70 nm) were cut on a Leica UCT ultramicrotome with a Diamond knife (Diatome), mounted on formvar-coated copper grids (Electron Microscopy Science), and counterstained with uranyl acetate and lead citrate. Random electron micrographs (35 per animal) were taken from the lesions with a FEI Spirit transmission microscope with a Gatan Orius SC200B Camera. A total of 3399 control axons and 2076 axons from Treg-depleted lesions were analysed blindly.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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2

Ultrastructural Analysis of Spinal Cord Lesions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animals were perfused with 3% glutaraldehyde, 2% paraformaldehyde, 0.1M phosphate buffer (pH 7.4), 0.7% (w/v) NaCl and spinal cords were dissected and post-fixed overnight. Specimen were post-fixed in 1% osmium tetraoxide (Agar Scientific, Stansted, UK), dehydrated, stained en bloc with uranyl acetate (Agar Scientific, Stansted, UK), and embedded in Durcupan resin (Sigma-Aldrich, UK). Ultrathin sections (70 nm) were cut on a Leica UCT ultramicrotome with a Diamond knife (Diatome), mounted on formvar-coated copper grids (Electron Microscopy Science), and counterstained with uranyl acetate and lead citrate. Random electron micrographs (35 per animal) were taken from the lesions with a FEI Spirit transmission microscope with a Gatan Orius SC200B Camera. A total of 3399 control axons and 2076 axons from Treg-depleted lesions were analysed blindly.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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