Originpro 8.5.0 sr1

MTT assays were performed in order to evaluate changes in the metabolic activity of cells that had occurred as a consequence of treatment with the acridine compounds. A549 cells (15 × 10³ cells/cm²) and CCD-18Co (15.625 × 10³ cells/cm²) were seeded in 96-well microplates. The A549 cells were treated for 24 and 48 h, and the CCD-18Co cells were treated for 48 h with different concentrations (5, 25, 50 and 75 µM) of the derivatives. After the treatment, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide) solution in PBS (5 mg mL⁻¹) was added to each well. The reaction was stopped after 4 h incubation, and the formazan was dissolved by the addition of SDS at a final concentration of 3.3%. The absorbance of formazan (λ = 584 nm) was measured using a BMG FLUOstar Optima spectrometer (BMG Labtech GmbH, Offenburg, Germany). The results were evaluated as percentages of the absorbance of the untreated control. Results are presented as the average percentage of cells from three independent experiments. IC₅₀ values for the derivatives were extrapolated from a sigmoidal fit (dose-response curve) to the metabolic activity data using OriginPro 8.5.0 SR1 (OriginLab Corp., Northampton, MA, USA).

All mathematical and statistical computations were made using Excel 2007 (Microsoft Office) and OriginPro 8.5.0 SR1 (OriginLab Corporation, USA). Data were reported as mean ± SD. Student t test was used for comparison of the developed method with standard method.

In this work, all experiments and measurements were carried out in triplicate, and data were expressed as a mean ± standard deviation (SD). The resulting data were analyzed by the use of OriginPro 8.5.0 SR1 (OriginLab Corporation, Northampton, MA, USA).

All analyses were completed using MedCalc version 20.010 (MedCalc Software Ltd., Ostend, Belgium) and OriginPro 8.5.0 SR1 (OriginLab Corporation, Northampton, MA, USA). Descriptive statistics were used to characterize the variables. A one-way repeated measures analysis of variances (ANOVA) followed by a Tukey post hoc test was used to determine whether three group means within each study group were different. Student’s t-test (unpaired two-sample test) was performed to test differences between groups at each follow-up. For comparison of nominal variables, the X² test for a 2 × 2 or higher contingency table was used. p values less than 0.05 were considered statistically significant.

MTT assays were performed in order to evaluate changes in the metabolic activity of cells that had occurred as a consequence of treatment with the biscoumarin derivatives. A549 cells (15 × 10³ cells/cm²) and CCD-18Co (15 625 × 10³ cells/cm²) were seeded in 96-well microplates. The cells were treated for 24 and 48 h with different concentrations (10, 25, 35, 50, 65, 75, and 100 µM) of the derivatives. After the treatment, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide) solution in PBS (5 mg mL⁻¹) was added to each well. The reaction was stopped after 4 h incubation, and the formazan was dissolved by the addition of sodium dodecyl sulfate (SDS) at a final concentration of 3.3%. The absorbance of formazan (λ = 584 nm) was measured using a BMG FLUOstar Optima spectrometer (BMG Labtech GmbH, Offenburg, Germany). The results were evaluated as percentages of the absorbance of the untreated control. Results are presented as the average percentage of cells from three independent experiments. IC₅₀ values for the derivatives were extrapolated from a sigmoidal fit (dose–response curve) to the metabolic activity data using OriginPro 8.5.0 SR1 (OriginLab Corp., Northampton, MA, USA).

Experimental data were analyzed for statistical significance by analysis of variance (ANOVA) and Tukey’s test with a p < 0.05 significance level with the help of statistical software OriginPro 8.5.0 SR1 (OriginLab Corporation, Northampton, MA, USA).

The tensile strength and percentage of elongation at break were determined using the methodology proposed by Fonseca-García, Jiménez-Regalado [11 (link)]. The experimental data regarding the mechanical properties were analyzed using OriginPro 8.5.0 SR1 software (OriginLab Corporation, Northampton, MA, USA) through an analysis of variance and the Tukey test with a significance level of p < 0.05.