Two days post-transduction, HeLa cells were trypsinized, resuspended in fresh culture medium and subsequently placed back in the incubator for 24 h before imaging. The DNA dye Hoechst 33342 (Thermo Scientific Cat. Nr: 62249) was diluted 1:10.000 in the culture medium, and the cells were then incubated at 37 °C in 10% CO2 for 10 min. Total and transgene-positive cells present in randomly selected microscopy fields were detected through fluorescence microscopy directed to the DNA dye and EGFP, respectively, by using an Olympus IX51 inverse fluorescence microscope equipped with an XC30 Peltier CCD camera and the CellF software (both from Olympus). Myoblasts were transduced as described above, and at 3 days post-transduction, the myoblasts were tripsinized and transferred to wells of 24-well plates. Bright-field images were acquired 2 days after re-seeding the cells by using an inverted Leica DMi8 microscope equipped with a DFC 450c camera, 40 × objective and the LAS 4.8.0 software (both from Leica).
Xc30 peltier ccd camera
Manufactured by Olympus
The XC30 Peltier CCD camera is a scientific imaging device that utilizes a Peltier-cooled charge-coupled device (CCD) sensor. The camera is designed to capture high-quality images and video for a variety of scientific and research applications.
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Lab products found in correlation
2 protocols using xc30 peltier ccd camera
1
Quantifying Transgene Expression in HeLa and Myoblast Cells
2
Visualizing eGFP Knockout in H27 Cells
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Targeted eGFP knockout in H27 cell cultures was monitored by direct fluorescence microscopy. The H27 cell nuclei were stained by adding Hoechst 33342 (Molecular Probes) at 10 μg/ml for 10 min. Next, the cell cultures were washed twice with PBS, after which culture medium was added. The eGFP- and Hoechst 33342-specific signals were detected by using an Olympus IX51 inverse fluorescence microscope (Olympus). Micrographs were captured and archived by deploying a XC30 Peltier CCD camera and the Cell̂F software (both from Olympus).
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