Log In Sign up
The largest database of trusted experimental protocols

Phospho akt ser473 193h12

Manufactured by Cell Signaling Technology
Visit Supplier

Phospho-AKT-Ser473 (193H12) is a primary antibody that specifically recognizes the Ser473-phosphorylated form of the AKT protein. AKT, also known as Protein Kinase B (PKB), is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes, including cell proliferation, survival, and metabolism.

Automatically generated - may contain errors

Lab products found in correlation

Superfrost plus slides, by Leica (2 mentions) Cryostat, by Leica (2 mentions) Mouse neuronal class β 3 tubulin clone tuj1, by Fortrea (2 mentions) Fluoromount g, by Southern Biotech (2 mentions) Pten 138g6, by Cell Signaling Technology (1 mentions) Phospho histone h2a x ser139 jbw301, by Merck Group (1 mentions) Igf 1rβ, by Cell Signaling Technology (1 mentions) Phospho marcks, by Cell Signaling Technology (1 mentions) Primary antibodies against phospho erk1 2, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Phospho-Akt (Ser473) (193H12) rabbit mAb Phospho-Akt (193H12), Phospho-Akt (Ser473) (Ser473) Akt Phospho-Akt

4 protocols using phospho akt ser473 193h12

1

Probing Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were purchased from Cell Signaling Technology: PTEN (138G6) (#9559), Akt (#9272), phospho-Akt (Ser473) (193H12) (#4058). Phospho-p27Kip1 (phospho T157) was purchased from abcam (ab85047). Phospho-Histone H2A.X (Ser139) (JBW301) (05-636) was from Millipore. Antibody against 53BP1 (A300-272A) was from Bethyl Laboratories.
Bassi C., Fortin J., Snow B.E., Wakeham A., Ho J., Haight J., You-Ten A., Cianci E., Buckler L., Gorrini C., Stambolic V, & Mak T.W. (2021). The PTEN and ATM axis controls the G1/S cell cycle checkpoint and tumorigenesis in HER2-positive breast cancer. Cell Death and Differentiation, 28(11), 3036-3051.
+ Open protocol
+ Expand
2

Retinal Tissue Preparation and Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols
4% PFA fixed eyes were dehydrated with increasing concentrations of sucrose solution (15%–30%) overnight and embedded in OCT on dry ice. Serial cross sections (12 μm) were cut with a Leica cryostat and collected on Superfrost Plus Slides. The retina sections were blocked in the staining buffer (10% normal goat serum (NGS) and 0.1% Triton X-100 in PBS) for 30 minutes followed by primary antibody staining. The following antibodies were used: mouse neuronal class β-III tubulin (clone Tuj1, 1:500 dilution; Covance), phospho-AKT-Ser473 (193H12, 1:200 dilution; Cell Signaling). The sections were incubated with primary antibodies overnight at 4°C and washed three times for 15 minutes each with PBS. Secondary antibodies (Cy2, Cy3 conjugated) were then applied (1:200–400; Jackson ImmunoResearch) and incubated for 1 hour at room temperature. Sections were again washed 3 times for 15 minutes each with PBS before a cover slip was attached with Fluoromount-G (Southernbiotech).
Yang L., Miao L., Liang F., Huang H., Teng X., Li S., Nuriddinov J., Selzer M.E, & Hu Y. (2014). The mTORC1 Effectors S6K1 and 4E-BP Play Different Roles in CNS Axon Regeneration. Nature communications, 5, 5416.
+ Open protocol
+ Expand
3

Retinal Tissue Preparation and Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols
4% PFA fixed eyes were dehydrated with increasing concentrations of sucrose solution (15%–30%) overnight and embedded in OCT on dry ice. Serial cross sections (12 μm) were cut with a Leica cryostat and collected on Superfrost Plus Slides. The retina sections were blocked in the staining buffer (10% normal goat serum (NGS) and 0.1% Triton X-100 in PBS) for 30 minutes followed by primary antibody staining. The following antibodies were used: mouse neuronal class β-III tubulin (clone Tuj1, 1:500 dilution; Covance), phospho-AKT-Ser473 (193H12, 1:200 dilution; Cell Signaling). The sections were incubated with primary antibodies overnight at 4°C and washed three times for 15 minutes each with PBS. Secondary antibodies (Cy2, Cy3 conjugated) were then applied (1:200–400; Jackson ImmunoResearch) and incubated for 1 hour at room temperature. Sections were again washed 3 times for 15 minutes each with PBS before a cover slip was attached with Fluoromount-G (Southernbiotech).
Yang L., Miao L., Liang F., Huang H., Teng X., Li S., Nuriddinov J., Selzer M.E, & Hu Y. (2014). The mTORC1 Effectors S6K1 and 4E-BP Play Different Roles in CNS Axon Regeneration. Nature communications, 5, 5416.
+ Open protocol
+ Expand
4

Western Blot Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies against phospho-ERK1/2 (rabbit monoclonal), ERK1/2, phospho-Akt (Ser473) (193H12; rabbit monoclonal), Akt, phospho-CREB (rabbit), phospho-MARCKS, CREB, IGF-1Rβ, β-actin, and Fmrp, as well as horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signaling Technology (Shanghai, China). Antibodies against PKCα and PKCβ were from Santa Cruz Biotechnology.
Zhang W., Xu C., Tu H., Wang Y., Sun Q., Hu P., Hu Y., Rondard P, & Liu J. (2015). GABAB receptor upregulates fragile X mental retardation protein expression in neurons. Scientific Reports, 5, 10468.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!