Fetal bovine serum fbs

Vero cells (ATCC CCL-81, Manassas, Virginia, United States) were cultivated in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Microtech, Naples, Italy) supplemented with a penicillin/streptomycin solution (Himedia, Naples, Italy) and 10% Fetal Bovine Serum (FBS, Microtech). HSV-1 (strain SC16), containing a lacZ gene driven by the cytomegalovirus IE-1 promoter to express beta-galactosidase, was grown on a Vero cell monolayer, as previously reported [29 (link)]. An acyclovir-resistant HSV-1 strain was selected in Vero cells receiving suboptimal acyclovir (ACV, Sigma-Aldrich) treatment.

Lipids were bought from Avanti Polar Lipids: HEPC (Egg, Chicken, 840,059); CHO (700,000); DSPE-PEG (880,120). Materials bought from Merck: Dulbecco′s Phosphate Buffered Saline (DPBS), (D1408); AM (A8423); deuterated chloroform (151,823); benzoic acid (TraceCERT); penicillin–streptomycin (P4333). Materials bought from Sigma Aldrich: Poly (2-hydroxyethyl methacrylate) (Poly-HEMA) (P3932); Ethanol (V001229); sodium orthovanadate (S6508); sodium fluoride (S6776); NaCl (S9888); rhodamine B 10 mg/ml (83,689). Cell culture media RPMI 1640 and trypsin (25,200,072) were acquired from ThermoFisher Gibco, while Hoechst (33,342) was bought from Thermo Fisher Scientific. Fetal bovine serum (FBS) was taken from Microtech (Napoli,Italy). Protease Inhibitor Cocktail was bought from Roche (04,693,116,001), secondary antibodies from Invitrogen (Goat anti-Rabbit 1gG 32,460, Rabbit anti- Goat IgG 31,402), CPT1A (D3B3) Rabbit mAb from Cell Signaling Technology (12,252) and vinculin Goat mAb from Santa Cruz (sc-7649). Protein Assay Dye was taken from Bio-Rad (5,000,006), Sample buffer 1X from GenScript (MB01015) and LiteAblot^® EXTEND Chemiluminescent Substrate from Euroclone (EMP013001). Water was purified with Milli-Q^® (Millipak^® 0.22 μm) system.