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Fibronectin coated glass bottom μ slide 8 well high

Manufactured by Ibidi
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The Fibronectin-coated glass-bottom μ-Slide 8-well high is a laboratory equipment product. It is a multi-well cell culture slide with a glass bottom and a fibronectin coating.

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Lab products found in correlation

Lipopolysaccharides (lps), by Enzo Life Sciences (2 mentions)

Close spelling variants of this product

μ-Slide 8 Well high Glass-bottom μ-slides Glass-bottom slides High glass bottom µ-Slide 8 Well μ-slides Fibronectin coated Glass slides Fibronectin

2 protocols using fibronectin coated glass bottom μ slide 8 well high

1

Isolation and Stimulation of Bone Marrow-Derived Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow-derived macrophages (BMDMs) were obtained by culturing the bone marrow monocytes from femurs of homozygous green-red DKI mice (9–12 weeks age) in M-CSF containing media for 6 days. BMDMs were re-plated in fibronectin-coated glass-bottom μ-Slide 8-well high (ibidi) on day 6. Coated glass-bottom surface was prepared by incubating 5 μg/cm2 plasma fibronectin (Fisher Scientific, cat# FC010) for 1hr at 37°C. The next day, BMDMs were stimulated with 10 ng/mL of lipopolysaccharide (LPS, Enzo Life Science) or murine TNF-α (R&D Systems, Cat#410-MT-010).
Rahman S.M., Aqdas M., Martin E.W., Ardori F.T., Songkiatisak P., Oh K.S., Uderhardt S., Yun S., Claybourne Q.C., McDevitt R.A., Greco V., Germain R.N., Tessarollo L, & Sung M.H. (2022). Double knockin mice show NF-κB trajectories in immune signaling and aging. Cell reports, 41(8), 111682.
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2

Isolation and Stimulation of Bone Marrow-Derived Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow-derived macrophages (BMDMs) were obtained by culturing the bone marrow monocytes from femurs of homozygous green-red DKI mice (9–12 weeks age) in M-CSF containing media for 6 days. BMDMs were re-plated in fibronectin-coated glass-bottom μ-Slide 8-well high (ibidi) on day 6. Coated glass-bottom surface was prepared by incubating 5 μg/cm2 plasma fibronectin (Fisher Scientific, cat# FC010) for 1hr at 37°C. The next day, BMDMs were stimulated with 10 ng/mL of lipopolysaccharide (LPS, Enzo Life Science) or murine TNF-α (R&D Systems, Cat#410-MT-010).
Rahman S.M., Aqdas M., Martin E.W., Ardori F.T., Songkiatisak P., Oh K.S., Uderhardt S., Yun S., Claybourne Q.C., McDevitt R.A., Greco V., Germain R.N., Tessarollo L, & Sung M.H. (2022). Double knockin mice show NF-κB trajectories in immune signaling and aging. Cell reports, 41(8), 111682.
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