Autoradiography was used to directly image the radiation emitted from the 18F-FDG tissue samples with high spatial resolution [23] (link) as further validation. Samples were placed in contact with a super resolution storage-phosphor screen (12.5×25.2 cm, PerkinElmer Inc., MA, USA). Over a sufficient exposure time (48 hours), emitted beta particles were recorded as an image on the storage-phosphor screen. The film was then read out by a Cyclone reader using a spatial resolution of 600 dpi (PerkinElmer Inc., Waltham, MA, USA). Using these techniques, the spatial distribution of radioisotope uptake of the tissue sample was recorded [24] .
Cyclone reader
Manufactured by PerkinElmer
Sourced in United States
The Cyclone reader is a lab equipment product from PerkinElmer. It is designed to detect and quantify luminescent signals in microplates, supporting various applications in life science research and analysis.
Automatically generated - may contain errors
2 protocols using cyclone reader
1
Autoradiography for Spatial Distribution
2
SAXS Characterization of Materials
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Scattering experiments were carried out using a W 3830 X-ray generator (PANalytical Co., Ltd., Almedo, Netherlands) and diffraction patterns were recorded using a SAXSess camera (Anton Paar Co., Ltd., Graz, Australia) in a line collimation system. A semitransparent beam stop was available to attenuate the beam. The samples were placed in a vacuum-proof metallic cell between Mylar windows, and the cell was tightened at both ends. Each sample was exposed to radiation of λ＝0.154 nm for 20 minutes in a vacuum, and the scattering pattern was detected on a 2D imaging plate. A cyclone reader (PerkinElmer Inc., Massachusetts, USA) was used to read the scattering patterns, which were converted to 1D profiles using SAXSquant software (Anton Paar Co., Ltd.) . All data were normalized to the same incident primary beam intensity 18, 23) .
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