In mir 145 5p

The small interfering RNA against circANKRD11 (si-circANKRD11), miR-145-5p mimics (miR-145-5p), miR-145-5p inhibitors (in-miR-145-5p), and control groups (si-NC, miR-NC and in-miR-NC) were provided by GenePharma (Shanghai, China). The overexpression plasmid of BRD4 (BRD4), the wild-type (WT) vectors of circANKRD11 (circANKRD11 WT) and the 3ʹ-untranslated region (3ʹUTR) of BRD4 (BRD4 3ʹUTR WT), the mutant (MUT) vectors of circANKRD11 (circANKRD11 MUT) and BRD4 3ʹUTR (BRD4 3ʹUTR MUT) and pcDNA 3.1 (+) (pcDNA) were obtained from Geneseed Co., Ltd. (Guangzhou, China). Constructed plasmids or synthesized oligonucleotides mixed with TurboFect reagent (Thermo Fisher, Waltham, MA, USA) were incubated with wells according to the instruction of manufacture. The sequences of synthesized oligonucleotides were displayed as below. Si-circANKRD11 5ʹ-GACACAGCAGACGGTTGATGA-3ʹ; miR-145-5p 5ʹ-GUCCAGUUUUCCCAGGAAUCCCU-3ʹ; in-miR-145-5p 5ʹ-AGGGAUUCCUGGGAAAACUGGAC-3ʹ; si-NC 5ʹ-CCTCTACCTGTCGCTGAGCTGTAAT-3ʹ; miR-NC 5ʹ-UUUGUACUACACAAAAGUACUG-3ʹ and in-miR-NC 5ʹ-CAGUACUUUUGUGUAGUACAAA-3ʹ.

Small interfering (si) RNA against circDNM3OS (si-circDNM3OS: 5ʹ-ATACTCTTTGTTTTGCACACT-3ʹ) and matching negative control (NC) (si-NC: 5ʹ-TTCTCCGAACGTGTCACGT-3ʹ), short hairpin (sh) RNA targeting circ-circDNM3OS (sh-circDNM3OS:5ʹ-CACCATACTCTTTGTTTTGCACACTCTCGAGAGTGTGCAAAACAAAGAGTA-3ʹ) and its NC (sh-NC: 5ʹ-UUCUCCGAACGUGUCACGUTT-3ʹ), miR-145-5p mimic (miR-145-5p) and corresponding NC (miR-NC), and miR-145-5p inhibitor (in-miR-145-5p) and matching NC (in-miR-NC) were acquired from GenePharma (Shanghai, China). The pcDNA-MORC2 (MORC2) vectors were constructed using the pcDNA vectors (Biovector Science Lab, Inc.). Transient transfection was executed using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA).