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Rat anti mouse cd16 cd32 mab

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The Rat anti-mouse CD16/CD32 mAb is a monoclonal antibody that binds to the mouse CD16 and CD32 receptors. These receptors are involved in the regulation of immune cell functions.

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Lab products found in correlation

Fixation permeabilization solution kit, by BD (3 mentions) Symphony a5 cytometer, by BD (3 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Gemini Bio (1 mentions) Cd11b, by BD (1 mentions) Paraformaldehyde (pfa), by Thomas Scientific (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Facscanto flow cytometer, by BD (1 mentions)

Spelling variants of this product

Anti-CD16/CD32 (48 citations) Anti-mouse CD16/CD32 (27 citations) CD16/CD32 (9 citations) Anti-mouse CD16/CD32 mAb (4 citations) Anti-CD16/CD32 mAb (4 citations) Rat anti-mouse CD16/CD32 (3 citations)

4 protocols using rat anti mouse cd16 cd32 mab

1

Multiparametric Flow Cytometry Analysis

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Splenocytes and siLPLs were incubated with a rat anti-mouse CD16/CD32 mAb (BioLegend, USA) for 30 min to block Fc receptors. Then, the cells were stained on ice with fluorochrome-conjugated monoclonal antibodies for 30 min as previously described [24 (link)]. To perform intracellular staining, cells were treated using a Fixation/Permeabilization Solution kit (BD Pharmingen™, USA) and then stained with the appropriate antibodies. The antibodies used in this study are listed in Additional file 2: Figure S6. Cells were assayed with a Symphony A5 cytometer (BD Biosciences), and the resulting data was analysed using FlowJo (ver. 10.4).
Liu H., Tian R., Wang H., Feng S., Li H., Xiao Y., Luan X., Zhang Z., Shi N., Niu H, & Zhang S. (2020). Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation. Journal of Translational Medicine, 18, 382.
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2

Identification of Dendritic Cell Subsets

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Following harvesting, pDCs and cDCs were incubated with rat anti-mouse CD16/CD32 mAb (Biolegend) for 15 min on ice to block FcγRs. Cells were then stained for 30 min in the dark on ice using specific antibodies for surface identification markers CD11c (eBioscience), CD11b (BD Bioscience), and B220 (StemCell Technologies), and costimulatory marker CD86 and CD40 (BD Bioscience). Cells were analyzed for cell viability using Fixable Viability Dye eFluor 780 (Thermo Fisher). Cells were fixed in 2% paraformaldehyde (Thomas Scientific) in PBS (Fisher) with 1% BSA (Gemini Bio Products). All cells were analyzed on a FACSCanto flow cytometer (BD Bioscience) with FlowJo software (Tree Star, Ashland, OR, USA).
Reznik G.O., Vajda S., Sano T, & Cantor C.R. (1998). A streptavidin mutant with altered ligand-binding specificity. Proceedings of the National Academy of Sciences of the United States of America, 95(23), 13525-13530.
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3

Immune Cell Phenotyping by Flow Cytometry

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Splenocytes and LPLs were incubated with a rat anti-mouse CD16/CD32 mAb (BioLegend, USA) for 30 min to block Fc receptors. Then, the cells were stained on ice with fluorochrome-conjugated monoclonal antibodies for 30 min as previously described [34] . To perform intracellular staining, cells were treated using a Fixation/Permeabilization Solution kit (BD Pharmingen™, USA) and then stained with the appropriate antibodies. The antibodies used in this study are listed in supplementary file table S2. Cells were assayed with a Symphony A5 cytometer (BD Biosciences), and the resulting data was analysed using FlowJo (ver. 10.4).
Ma Y., Guo R., Sun Y., Li X., He L., Zhao L., Silverman G.J., Chen G., Gao F., Yuan J., Qiang W., Li M., Lu L., & Niu H. (2021). Lupus Gut Microbiota Transplants Cause Autoimmunity and Inflammation.
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4

Immune Cell Phenotyping by Flow Cytometry

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Splenocytes and LPLs were incubated with a rat anti-mouse CD16/CD32 mAb (BioLegend, USA) for 30 min to block Fc receptors. Then, the cells were stained on ice with fluorochrome-conjugated monoclonal antibodies for 30 min as previously described [34] . To perform intracellular staining, cells were treated using a Fixation/Permeabilization Solution kit (BD Pharmingen™, USA) and then stained with the appropriate antibodies. The antibodies used in this study are listed in supplementary file table S2. Cells were assayed with a Symphony A5 cytometer (BD Biosciences), and the resulting data was analysed using FlowJo (ver. 10.4).
Ma Y., Guo R., Sun Y., Li X., He L., Li Z., Silverman G.J., Chen G., Gao F., Yuan J., Wei Q., Li M., Lu L, & Niu H. (2021). Lupus gut microbiota transplants cause autoimmunity and inflammation. Clinical immunology (Orlando, Fla.), 233.
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