Luxscan 10 k a laser confocal scanner

The analysis of miRNA microarray data was conducted in 32 clinical samples acquired from lung adenocarcinoma patients with BM in contrast to 55 patients without BM obtained from the Cancer Hospital, Chinese Academy of Medical Sciences (Beijing, China) between 2003 and 2008. In short, total RNA isolated from patient samples was examined with the mammalian miRNA array V2.0 (CapitalBio, Beijing, China), which identifies 1105 miRNAs in humans, mice, and rats. Separation of low-molecular-weight RNAs from total RNA was carried out by a PEG precipitation method; the low-molecular-weight RNAs were then labeled with 5-phosphate-cytidyl-uridyl-Cy3–3 and then hybridized to the mammalian miRNA array overnight at 42 °C. A LuxScan 10 K/A laser confocal scanner was used to scan the arrays, and the acquired images were evaluated using LuxScan 3.0 software (both from CapitalBio). Cluster 3.0 was used to carry out clustering analysis, and the results were viewed with TreeView software. The normalization of fluorescence signals was carried out using the median center tool for genes in Cluster 3.0, and they were evaluated using the significance analysis of microarrays (SAM), with a false discovery rate (FDR) threshold set of 0 and fold-change established at ≥2- or ≤ 0.5-fold change and p value < 0.05.

The analysis of miRNA microarray data was conducted in 32 clinical samples acquired from LAD patients with BM in contrast to 55 patients without BM. In short, total RNA isolated from patient samples was examined with the mammalian miRNA array V2.0 (CapitalBio, Beijing, China), which identified 1105 miRNAs in humans, mice, and rats. Separation of low-molecular-weight RNAs from total RNA was conducted by the polyethylene glycol precipitation method; then low-molecular-weight RNAs were labeled with 5-phosphate-cytidyl-uridyl-Cy3-3 and hybridized to the mammalian miRNA array overnight at 42 °C. The LuxScan 10 K/A laser confocal scanner was used to scan the arrays, and the acquired images were evaluated using LuxScan 3.0 software (both from CapitalBio). Cluster 3.0 was used to conduct the clustering analysis, and the results were viewed with TreeView software.