Cells were lysed and total protein was extracted with RIPA lysis buffer (Beyotime Technology). The total protein concentration was determined with a BCA kit (Thermo Fisher). The protein samples were boiled at 95°C for 5 min, separated by 10% SDS-PAGE, and transferred to 0.45 μm PVDF membranes (Millipore). After incubation with primary antibodies overnight at 4°C, these membranes were washed with TBST buffer and incubated with secondary antibodies at room temperature. The primary antibodies were SMARCC1 (ab172638, 1:1,000, Abcam, United Kingdom), KPNA2 (ab70160, 1:10,000, Abcam, United Kingdom), NUP50 (ab151567, 1:2000, Abcam, United Kingdom), NUP153 (ab171074, 1:1,000, Abcam, United Kingdom) and β-tubulin (ab6046, 1:5000, Abcam, United Kingdom). The secondary antibodies were anti-rabbit IgG (7074,1:2000, Cell Signaling Technology, United States) and anti-mouse IgG (7076, 1:2000, Cell Signaling Technology, United States) HRP linked antibody. Immunoreactive bands were visualized by enhanced chemiluminescence using HRP Substrate Peroxide (Millipore).
Ab151567
Manufactured by Abcam
Sourced in United Kingdom
Ab151567 is a laboratory equipment product. It serves a core function as a tool for research and analysis in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Beyotime (1 mentions)
Bca kit, by Thermo Fisher Scientific (1 mentions)
Ab172638, by Abcam (1 mentions)
Ab6046, by Abcam (1 mentions)
Ab70160, by Abcam (1 mentions)
Ab171074, by Abcam (1 mentions)
Mouse anti v5, by Thermo Fisher Scientific (1 mentions)
Rabbit anti foxo1, by Cell Signaling Technology (1 mentions)
Mouse anti gata4, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti parp, by Cell Signaling Technology (1 mentions)
Rabbit anti nup98, by Cell Signaling Technology (1 mentions)
Mouse anti oct3 4, by Santa Cruz Biotechnology (1 mentions)
Z0334, by Agilent Technologies (1 mentions)
Ab32423, by Abcam (1 mentions)
Ab12073, by Abcam (1 mentions)
Ab21873, by Abcam (1 mentions)
Mouse anti α tubulin, by Merck Group (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
Goat anti oct3 4, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti sox2, by Cell Signaling Technology (1 mentions)
2 protocols using ab151567
1
Western Blot Analysis of Nuclear Proteins
2
Antibody Characterization for Cell Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The primary antibodies used included mouse anti-Nup153, ascites fluid (gift from B. Burke), rabbit anti-Nup153 (raised against hNup153-GST, amino acids 1300–1399), rabbit anti-Parp (9542, Cell Signaling), mouse anti-Oct-3/4 (sc-5279, Santa Cruz Biotechnology), goat anti-Oct-3/4 (sc-8628, Santa Cruz Biotechnology), mouse anti-α-tubulin (T5168, Sigma-Aldrich), mouse anti-Flag (F1804, Sigma-Aldrich), rabbit anti-Nup107 (raised against mNup107-His, amino acids 600–926), rabbit anti-Tuj1 (neuron-specific class III β-Tubulin; PRB-435P, BioLegend), mouse anti-Gapdh (GTX627408, Genetex), rabbit anti-Blbp (Fabp7; ab32423, Abcam), rat anti-Nestin (556309, BD pharmigen), mouse anti-Gata4 (sc-25310, Santa Cruz Biotechnology), rabbit anti-GFAP (Z0334, Dako), goat anti-Foxa2 (AF2400, R&D Systems), mouse anti-α-Sma (A5228, Sigma), rabbit anti-Nup98 (2292, Cell Signaling), rabbit anti-Sox2 (2748, Cell Signaling), mouse anti-V5 (46-0705, Life Technologies), mouse anti-Ring1b (39663, Active Motif), rabbit anti-Ring1b (5694S, Cell Signaling), rabbit anti-Rybp (ab5976, Abcam), rabbit anti-Cbx7 (ab21873, Abcam), rabbit anti-Nup50 (ab151567, Abcam), rabbit anti-Suz12 (ab12073, Abcam), rabbit anti-Lmnb2 (ARP46356; Aviva Systems Biology), rabbit anti-Mel-18 (sc-10744, Santa Cruz Biotechnology), and rabbit anti-Foxo1 (2880S, Cell Signaling).
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