The GC–MS analysis of Illicium verum extract was carried out by using Agilent Technologies GC systems with GC-7890A/MS-5975C model (Agilent Technologies, Santa Clara, CA, USA) equipped with OPTIMA-5 column. Helium was used as a carrier gas with a flow rate of 1.0 ml/min. The temperature was set at 50 °C for 3 minutes with an increasing rate of 10 °C/min for 20 minutes and a holding time of about 20 min. Finally, the temperature was increased to 300 °C at 10 °C/min and was kept for 25 minutes. The mass spectrometer was operated in EI mode (70 eV). The extract was dissolved in ethanol, filtered, and then injected in a split mode [20 (link)]. The compounds were identified by comparison of their mass spectra with standards available in the NIST mass spectral library attached to the GC-MS instrument and the results obtained have been presented in Table 3 .
Gc 7890a ms 5975c model
Manufactured by Agilent Technologies
Sourced in United States
The GC-7890A/MS-5975C is a gas chromatograph-mass spectrometer (GC-MS) system manufactured by Agilent Technologies. It is designed to separate, identify, and quantify chemical compounds in complex mixtures. The GC-7890A component is responsible for sample preparation and separation, while the MS-5975C component is used for the detection and analysis of the separated compounds.
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2 protocols using gc 7890a ms 5975c model
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GC-MS Analysis of Illicium verum Extract
2
GC-MS Analysis of Bioactive Compounds
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The GC-MS analysis of bioactive compounds from the different extracts of the leaves was done using Agilent Technologies GC systems with GC-7890A/ MS-5975C model (Agilent Technologies, Santa Clara, CA, USA) equipped with HP-5MS column (30 m in length × 250 μm in diameter × 0.25 μm in thickness of film). Spectroscopic detection by GC-MS involved an electron ionization system which utilized high energy electrons (70eV). Pure helium gas (99.995%) was used as the carrier gas with flow rate of 1 mL/min. The initial temperature was set at 50˚C -150˚C with increasing rate of 3˚C/min and holding time of about 10 min. Finally, the temperature was increased to 300˚C at 10˚C/min. One microliter of the prepared 1% of the extracts diluted with respective solvents was injected in a split-less mode. Relative quantity of the chemical compounds present in each of the extracts of B. luzonica was expressed as percentage based on peak area produced in the chromatogram.
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