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Hydroxybenzotriazole hobt

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Hydroxybenzotriazole (HOBt) is a heterocyclic organic compound that functions as a coupling agent in organic synthesis. It is used to facilitate the formation of amide bonds between amino acids during peptide synthesis.

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Lab products found in correlation

Anhydrous tetrahydrofuran thf, by Thermo Fisher Scientific (1 mentions) Anhydrous dimethylformamide dmf, by Thermo Fisher Scientific (1 mentions) Anhydrous ethanol, by Merck Group (1 mentions) Ethanolamine, by Merck Group (1 mentions) Triethylamine, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) Pyridine, by Thermo Fisher Scientific (1 mentions) Potassium carbonate k2co3, by Merck Group (1 mentions) 11 bromoundecanoic acid, by Merck Group (1 mentions) Poly dgdc poly dgdc, by Merck Group (1 mentions) Poly a poly u, by Merck Group (1 mentions) Triethylamine et3n, by Merck Group (1 mentions) Calf thymus dna, by Merck Group (1 mentions)

Spelling variants of this product

Hydroxybenzotriazole (20 citations) 1-hydroxybenzotriazole hydrate (HOBt) (12 citations) 1‐hydroxybenzotriazole (HOBt (7 citations) N-hydroxybenzotriazole (HOBt), (4 citations) N,N-dimethyl formamide (DMF), dichloromethane (DCM), 1-hydroxybenzotriazole (HOBt), trifluoroacetic acid (TFA), (2 citations)

2 protocols using hydroxybenzotriazole hobt

1

Rhodamine Fluorescent Probe Synthesis

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Materials were used as received: 2-(4-diethylamino-2-hydroxybenzoyl)benzoic
acid (98%, TCI America), resorcinol (1,3-benzene diol, 99%, Sigma-Aldrich),
trifluoroacetic acid (TFA, 99%, Sigma-Aldrich), ethanolamine (98%,
Sigma-Aldrich), anhydrous ethanol (99.5%, Sigma-Aldrich), 5-norbornene-2-carboxylic
acid (NBCOOH, mixture of endo- and exo-isomers, 98%, Sigma-Aldrich), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, 98%, Proteochem),
hydroxybenzotriazole (HOBt, 97%, Sigma-Aldrich), 11-bromoundecanoic
acid (99%, Sigma-Aldrich), potassium carbonate (K2CO3, 99%, Sigma-Aldrich), anhydrous dimethylformamide (DMF, 99%,
Acros Organics), dry methylene chloride (CH2Cl2, DCM, 99.8%, Acros Organics), anhydrous tetrahydrofuran (THF, 99.8%,
Acros Organics), triethylamine (99.5%, Sigma-Aldrich), pyridine (99.8%,
Acros Organics), second-generation Grubbs catalyst ((H2IMes)(pyr)2(Cl)2RuCHPh (mG2), 98%, AK Scientific),
and ethyl vinyl ether (EVE, 99%, Acros Organics). Rhodamine HO-Rh-OH
was synthesized using a literature protocol.8 (link)
Vaidya S., Sharma M., Brückner C, & Kasi R.M. (2021). Rhodamine-Installed Polynorbornenes: Molecular Design, Structure, and Stimuli-Responsive Properties. ACS Omega, 6(23), 15017-15028.
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2

Synthesis and Characterization of Pyrene-Derived Compounds

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Chemicals for synthesis: 4-(1-pyrenyl)butyric acid (Alfa Aesar, 97%), triethylamine (Et3N) (Sigma Aldrich, 99%), hydroxybenzotriazole (HOBt) (Sigma Aldrich, 97%), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) (Alfa Aesar, 98%), Silica gel 60, PF254 for preparative thin layer chromatography (Merck).
Nucleotides (AMP, GMP, UMP and CMP) were purchased from Sigma, dissolved in stock solutions of c = 0.01 M and used. Polynucleotides were purchased as noted: poly dAdT–poly dAdT, poly A–poly U, poly dGdC–poly dGdC, (Sigma), calf thymus (ct)-DNA (Aldrich) and dissolved in sodium cacodylate buffer (I = 0.05 M, pH = 7.0), as described by producer. The presence of double stranded helix was confirmed by single, well-defined transition in the thermal denaturation experiment, giving Tm value agreeing well with the literature; as well as by collecting CD spectra of free ds-DNA or ds-RNA, which also agreed well with the literature.48,49 (link) The ct-DNA was additionally sonicated and filtered through a 0.45 mm filter to obtain mostly short (ca. 100 base pairs) rod-like B-helical DNA fragments. Polynucleotide concentration was determined spectroscopically as the concentration of phosphates (corresponds to c(nucleobase)).52
Nikšić-Franjić I., Colasson B., Reinaud O., Višnjevac A., Piantanida I, & Pavlović Saftić D. (2023). Novel pyrene-calix[4]arene derivatives as highly sensitive sensors for nucleotides, DNA and RNA. RSC Advances, 13(39), 27423-27433.
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