We used the Drosophila Activity Monitoring System (DAM System, TriKinetics, Waltham, MA) to assess sleep and activity phenotypes. At 1–2 days of age, we placed flies into DAM tubes containing 2% agar with 5% sucrose, sealed with a rubber cap (TriKinetics), and a small piece of yarn. We collected data for 7 days on a 12-hour light-dark cycle, with sleep defined as at least 5 minutes of inactivity. We discarded data from flies that did not survive the entire testing period, leaving 18–32 flies per sex per line for analysis. We processed the raw sleep and activity data using ShinyR-DAM (Cichewicz and Hirsh 2018 (link)) and used the resulting output data for statistical analysis.
Rubber cap
Manufactured by Trikinetics
The Rubber Cap is a protective cover designed for laboratory equipment. It serves to seal and protect the components of the equipment, safeguarding them from external factors such as dust, moisture, and impacts.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rubber cap
1
Drosophila Sleep and Activity Phenotyping
2
Drosophila Activity Monitoring System: Sleep and Activity Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used the Drosophila Activity Monitoring System (DAM System, TriKinetics, Waltham, MA) to assess sleep and activity phenotypes. At 1-2 days of age, we placed flies into DAM tubes containing 2% agar with 5% sucrose, sealed with a rubber cap (TriKinetics) and a small piece of yarn. We collected data for 7 days on a 12-hour light-dark cycle, with sleep defined as at least 5 minutes of inactivity. We discarded data from flies that did not survive the entire testing period, leaving 18-32 flies per sex per line for analysis. We processed the raw sleep and activity data using ShinyR-DAM (Cichewicz and Hirsh 2018 (link)) and used the resulting output data for statistical analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!