Secondary metabolites present in the leaves and flowers extracts of L. camara cultivars were determined using an UPLC XEVO TQD triple quadruple instrument Waters Corporation, Milford, MA01757 U.S.A. The chromatographic system was comprised of a Waters Acquity QSM pump, a LC-2040 autosampler, degasser in addition to Waters Acquity CM detector. The dimensions of Waters Acquity UPLC BEH C18 column that was used for the chromatographic separation were: 50 mm (length), 2.1 mm (internal diameter) and 1.7 μm (particle size). The operation of the column was at a flow rate of 0.2 mL/min and the system was thermostated at 30 °C.
The mobile phase, that was used for analysis, was composed of two phases; phase A and B. Phase A was composed of ultrapure water +0.1% formic acid, while phase B consisted of methanol +0.1% formic acid. These mobile phase components were used in order to provide good separation and resolution for the compounds. Moreover, 0.1% formic acid is a suitable solvent regarding MS detectors, as it shows a distinct influence on the responses and ionization efficiency of analytes (Alberti-Dér, 2013 ). Elution was a gradient one and its program was as following: 0.0–2.0 min, 10% B; 2.0–5.0 min, 30% B; 5.0–15.0 min, 70% B; 22.0min, 90% B; 22.0–25.0 min, 90% B; 26.0 min, 100% B; 26.0–29.0 min, 100% B; 30.0 min, 10% B; followed by 4 min to re-equilibrate the column.
The mobile phase, that was used for analysis, was composed of two phases; phase A and B. Phase A was composed of ultrapure water +0.1% formic acid, while phase B consisted of methanol +0.1% formic acid. These mobile phase components were used in order to provide good separation and resolution for the compounds. Moreover, 0.1% formic acid is a suitable solvent regarding MS detectors, as it shows a distinct influence on the responses and ionization efficiency of analytes (Alberti-Dér, 2013 ). Elution was a gradient one and its program was as following: 0.0–2.0 min, 10% B; 2.0–5.0 min, 30% B; 5.0–15.0 min, 70% B; 22.0min, 90% B; 22.0–25.0 min, 90% B; 26.0 min, 100% B; 26.0–29.0 min, 100% B; 30.0 min, 10% B; followed by 4 min to re-equilibrate the column.