The absorption spectra of Cu2+ containing solutions were recorded on a Varian Cary50 Probe spectrophotometer, in the range 350–900 nm, using a quartz cuvette with an optical path of 1 cm. To describe the species present in solution, the observed wavelength of maximum absorption at a given pH was compared with the expected λmax value obtained from literature23 (link),48 (link)–50 (link). Circular dichroism (CD) spectra were recorded on a Jasco J-1500 spectropolarimeter in the 180–800 nm range, using a quartz cuvette with an optical path of 1 cm in the visible and near-UV range, and 0.01 cm in the 180–250 nm range. Electron paramagnetic resonance (EPR) spectra were recorded in liquid nitrogen on a Bruker ELEXSYS E500 CW-EPR spectrometer at X-band frequency (9.5 GHz) and equipped with an ER 036TM NMR teslameter and an E41 FC frequency counter. Ethylene glycol (30%) was used as a cryoprotectant. The EPR parameters were analyzed by computer simulation of the experimental spectra using WIN-EPR SIMFONIA software, version 1.2 (Bruker, Billerica, MA, USA).
Win epr simfonia software version 1
Manufactured by Bruker
Sourced in United States
WIN-EPR SIMFONIA software, version 1.2, is a product from Bruker. It is an application software used for the simulation of electron paramagnetic resonance (EPR) spectra.
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Lab products found in correlation
Elexsys e500 cw epr spectrometer, by Bruker (1 mentions)
Er 036tm nmr teslameter, by Bruker (1 mentions)
Cary 50 probe spectrophotometer, by Agilent Technologies (1 mentions)
J 1500 spectropolarimeter, by Jasco (1 mentions)
Continuous flow liquid helium cryostat, by Oxford Instruments (1 mentions)
Er 4102 t, by Oxford Instruments (1 mentions)
Uv 1800 spectrophotometer, by Shimadzu (1 mentions)
2 protocols using win epr simfonia software version 1
1
Spectroscopic Characterization of Cu2+ Solutions
2
Spectroscopic Characterization of Laz Protein
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The visible-spectra were recorded on a Shimadzu UV-1800 spectrophotometer using 1 cm quartz cells. Laz samples were oxidized with a solution of potassium ferricyanide and reduced with a solution of sodium ascorbate.
The Laz sample for EPR was 0.3 mM in 20 mM phosphate buffer, pH 7.0. The EPR spectra of Laz were recorded on a X-band Bruker EMX Fig. 1. Analysis of the primary sequence of Ng Laz. In green underlined is the signal peptide recognized by the signal peptidase II pathway; in blue are identified the residues of the mature protein recognized by the epitope for the H.8 MAb; and in black the azurin-like domain.
spectrometer equipped with a rectangular cavity (model ER 4102T) and an Oxford Instruments continuous liquid helium flow cryostat. Experimental conditions are described in the legend of the spectrum. The simulation of the spectrum was performed using WINEPR SimFonia software version 1.2, from Bruker.
The Laz sample for EPR was 0.3 mM in 20 mM phosphate buffer, pH 7.0. The EPR spectra of Laz were recorded on a X-band Bruker EMX Fig. 1. Analysis of the primary sequence of Ng Laz. In green underlined is the signal peptide recognized by the signal peptidase II pathway; in blue are identified the residues of the mature protein recognized by the epitope for the H.8 MAb; and in black the azurin-like domain.
spectrometer equipped with a rectangular cavity (model ER 4102T) and an Oxford Instruments continuous liquid helium flow cryostat. Experimental conditions are described in the legend of the spectrum. The simulation of the spectrum was performed using WINEPR SimFonia software version 1.2, from Bruker.
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