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Citric acid monohydrate

Manufactured by Beijing Chemical Works
Sourced in China

Citric acid monohydrate is a chemical compound commonly used as a pH regulator, preservative, and flavoring agent. It is a crystalline solid that is soluble in water and has a tart, acidic taste. Citric acid monohydrate is a versatile chemical with various industrial and commercial applications.

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3 protocols using citric acid monohydrate

1

Iridium-based Catalysts Synthesis

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Potassium hexachloroiridate(IV) (K2IrCl6, 99.99%) was purchased from Aladdin. Strontium nitrate (Sr(NO3)2), citric acid monohydrate (C6H8O7·H2O), ethylene glycol, hydrochloric acid (HCl) and sulfuric acid (H2SO4) were purchased from Beijing Chemical Factory. Cobalt nitrate hexahydrate (Co(NO3)2·6H2O) was purchased from Shantou Xilong Chemical Factory. Nafion® perfluorinated resin solution (5 wt.% in mixture of lower aliphatic alcohols and water containing 45% water) was purchased from Sigma-Aldrich. All the chemicals and reagents were used without further purification. Highly purified water (>18 MΩ cm resistivity) was provided by a PALL PURELAB Plus system.
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2

Analytical Reagents for Chemical Experimentation

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Crystalline iodine, potassium iodide, citric acid monohydrate, disodium hydrogen phosphate dodecahydrate, potassium bromide, sodium thiosulfate, concentrated sulfuric acid, soluble starch, potassium dihydrogen sulfate, disodium hydrogen phosphate, borax, and sodium hydroxide, were all obtained analytically pure from the Beijing Chemical Factory; soluble starch was obtained analytically pure from the Beijing Aoboxing Biotechnology Co., Ltd. (Beijing, China); α-amylase was obtained from the Beijing Aoboxing Biotechnology Co., Ltd. (Beijing, China); sodium indigo disulfonate was obtained form the Sinopharm Chemical Reagent Company BS (Beijing, China); sodium calcium reagent carboxylate was obtained from the Sinopharm Chemical Reagent Company Ind (Beijing, China); and malachite green was obtained from the Beijing Chemical Reagent Company AR (Beijing, China).
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3

Timm's Staining for Mossy Fiber Sprouting

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Timm’s staining was performed as previously described (Wang et al., 2016 ). Mice were anesthetized and perfused intracardially with 50 ml 0.9% NaCl (Sigma-Aldrich) solution, followed by 50 ml 4% paraformaldehyde solution (Sigma-Aldrich), 100 ml 1% sodium sulfide solution (Sigma-Aldrich), and 50 ml 4% paraformaldehyde solution again. Slices were stained in Timm’s developer solution for 110 min in darkness at room temperature. Timm’s developer solution was freshly made from 60 ml gum arabic (30 g; Vetec), 10 ml citric acid monohydrate (2.55 g; Beijing Chemical Works) and trisodium citrate dehydrate (2.35 g; Beijing Chemical Works), 15 ml hydroquinone (0.85 g; Sigma-Aldrich), and 15 ml silver nitrate (0.11 g; Sinopharm Chemical Reagent). Subsequently, sections were washed with distilled water and counterstained with hematoxylin (ZSGB-BIO). An investigator blinded to the treatment assessed the degree of mossy fiber sprouting into the supragranular layer of the DG. The standard for scoring was described previously (Wang et al., 2016 ).
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