ECH and prazosin (an antagonist of α1-adrenergic receptors [AR]) were acquired from Sigma-Aldrich (USA). The D1-like dopaminergic receptor agonist SKF38393 was obtained from Abcam (UK). SCH 23390 (DRD1/5 antagonist) and cirazoline (an α1-AR agonist) were purchased from Tocris Bioscience (UK). All other chemicals employed in this research were of analytical grade.
Skf38393
Manufactured by Abcam
Sourced in United Kingdom
SKF38393 is a dopamine D1 receptor agonist. It is used in pharmacological research to study the function and regulation of dopamine D1 receptors.
Automatically generated - may contain errors
Lab products found in correlation
Sch23390, by Bio-Techne (4 mentions)
Cirazoline, by Bio-Techne (2 mentions)
Prazosin, by Merck Group (2 mentions)
Apomorphine hydrochloride, by Merck Group (1 mentions)
Benserazide hcl, by Merck Group (1 mentions)
L dopa, by Abcam (1 mentions)
L dopa, by Merck Group (1 mentions)
Forskolin, by Fujifilm (1 mentions)
Modafinil, by Merck Group (1 mentions)
Pd 168077, by Bio-Techne (1 mentions)
7 azido 4 methylcoumarin, by Merck Group (1 mentions)
α smooth muscle actin α sma, by Proteintech (1 mentions)
P21cip waf 1, by Proteintech (1 mentions)
Edu cell proliferation assay kit, by RiboBio (1 mentions)
Fluo 4 am, by Beyotime (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
Enhanced ecl chemiluminescent substrate kit, by Yeasen (1 mentions)
Y 27632, by MedChemExpress (1 mentions)
Bromocriptine, by Bio-Techne (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Cell Signaling Technology (1 mentions)
6 protocols using skf38393
1
Adrenergic and Dopaminergic Receptor Modulation
2
Adrenergic and Dopaminergic Receptor Modulation
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ECH and prazosin (an antagonist of α1-adrenergic receptors [AR]) were acquired from Sigma-Aldrich (USA). The D1-like dopaminergic receptor agonist SKF38393 was obtained from Abcam (UK). SCH 23390 (DRD1/5 antagonist) and cirazoline (an α1-AR agonist) were purchased from Tocris Bioscience (UK). All other chemicals employed in this research were of analytical grade.
3
Neuroprotective Effects of GSK-3β Inhibition and D1 Agonism on L-dopa-induced Dyskinesia
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Apomorphine hydrochloride (Sigma-Aldrich, St. Louis, MO, USA) was administered (0.5 mg/kg). L-dopa (Sigma-Aldrich, 25 mg/kg) plus benserazide-HCl (Sigma-Aldrich, 6.25 mg/kg) were given once-daily. TDZD8, a non-ATP competitive inhibitor of GSK-3β, was dissolved in 10% DMSO and was administered i.p. (TDZD8-L group, 1 mg/kg; TDZD8-H group, 2 mg/kg, respectively) 30 min prior to L-dopa intake for 3 weeks. The dose of TDZD8 used was based on previous studies23 (link). (±)-1-Phenyl-2,3,4,5-tetrahydro-(1H)-3-benzazepine-7,8-diol hydrochloride (SKF38393, Abcam, UK), a D1 Dopamine receptor agonist, was dissolved in saline and was administered i.p. (SKF38393-L group, 5 mg/kg; SKF38393-H group, 10 mg/kg, respectively) 30 min prior to L-dopa intake for 3 weeks. The dose of TDZD8 used was based by Iderberg et al.24 (link). The dose of L -dopa (25 mg/kg) was chosen by our previous study25 (link).
4
Modulation of Retinal Dopamine Signaling
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Dopamine receptor agonist cocktail contained D1 receptor agonist (SKF38393; Abcam, Cambridge, UK) and D4 receptor agonist (PD168077; Tocris, Bristol, UK) dissolved in saline. Mice kept under SD conditions were i.p. injected with dopamine agonist cocktail (1 mg/kg) 1 h before the ERG recording under dim red light, as described previously18 (link). Dopamine receptor antagonist cocktail contained D1 receptor antagonist (SCH 23390; Tocris) and D4 receptor antagonist (L-745870; Abcam) (1 mg/kg each) dissolved in saline. Mice kept under LD conditions were i.p. injected with dopamine antagonist cocktail 1 h before ERG recording under dim red light. Based on previous reports46 (link),47 (link), modafinil (M6940; Sigma-Aldrich, St. Louis, MO, USA), dissolved in 0.1% DMSO/saline, was injected by gavage (64 mg/kg, 4 h apart, three times each day during daytime for 3 days). Forskolin (66575-29-9; Wako, Osaka) dissolved in saline (1%) was administered to the eyes (2 µl each) as eye-drops using a pipet (4 h apart, three times each day during daytime for 3 days). The effects of topical administration of Forskolin were examined twice by different experimenters, and consistent results were obtained in both experiments.
5
Molecular Mechanisms of Fibrosis Inhibition
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Sodium hydrogen sulfide (NaHS), PPG (a CSE inhibitor) and 7-Azido-4-Methylcoumarin (AzMC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). SKF38393 (a DR1 agonist) was obtained from Abcam (Cambridge, MA, USA). Y-27632 (a ROCK inhibitor) was obtained from MedChemExpress (Shanghai, China). The primary antibodies for anti-CSE, Cyclin D1, proliferating cell nuclear antigen (PCNA), p21 Cip/W AF -1 , collagen I (Col-1), collagen III (Col-3), matrix metalloproteinase 9 (MMP-9), osteopontin (OPN) and α-smooth muscle actin (α-SMA) were purchased from Proteintech (Wuhan, China). The anti-p-RhoA, t-RhoA, p-ROCK1, t-ROCK1 were from Affinity Biosciences (Cincinnati, OH, USA). The anti-DR1 antibody was from GeneTex (Irvine, CA, USA). Horseradish peroxidase-conjugated goat anti-rabbit IgG, goat anti-mouse IgG antibody, the Cell Counting Kit-8 (CCK-8) and anti-β-actin were obtained from Boster Bioengineering Limited Company (Wuhan, China). The EdU Cell Proliferation Assay Kit were obtained from Ribobio (Guangzhou, China). Fluo-4 AM were obtained from Beyotime Biotechnology (Shanghai, China). Enhanced ECL Chemiluminescent Substrate Kit was obtained from Yeasen Biotechnology (Shanghai, China). All other chemicals were from Solarbio (Beijing, China) or Beyotime Biotechnology.
6
Signaling Pathways in Adipocyte Metabolism
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Antibodies against peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1A) and the p38 mitogen-activated protein kinase (MAPK) inhibitor SB 202190 were purchased from Calbiochem. Phosphospecific p38 MAPK, total p38 MAPK and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were obtained from Cell Signalling. TOMM20 antibodies were purchased from Abnova (Taipei, Taiwan). ATP synthase beta antibodies and the specific D1-like agonist SKF 38393 were from Abcam. Specific UCP1 and D1-like receptor antibodies were purchased from Chemicon International. Specific D2-like receptor antibodies and the specific D2-like antagonist raclopride were from Santa Cruz Biotechnology. The specific D1-like antagonist SCH 23390 and D2-like agonist bromocriptine were purchased from Tocris Bioscience (Bristol, UK). Secondary antibodies were from Life Technologies. Fatty acid-free bovine serum albumin (BSA) was from Serva (Heidelberg, Germany). All other materials were obtained from Sigma-Aldrich.
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