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Cfi fluor 10x

Manufactured by Nikon
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The CFI Fluor 10X is a microscope objective lens developed by Nikon. It is designed to provide clear and detailed images when working with fluorescence microscopy techniques.

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Lab products found in correlation

Ff01 520 35 25, by IDEX Corporation (2 mentions) Figh 30 650s, by Nikon (2 mentions) Bl 2 473 1 100mw, by Thorlabs (2 mentions) F230fc a, by Thorlabs (2 mentions) Ff495 di02 25 36, by IDEX Corporation (2 mentions)

2 protocols using cfi fluor 10x

1

Multi-fiber Imaging with Laser Excitation

Check if the same lab product or an alternative is used in the 5 most similar protocols
A bundle of three imaging fibers with individual diameters of 0.6mm and core-to-core distance of 3.3 μm (Myriad Fibers, FIGH-30–650s) was coupled to the imaging objective (Nikon, CFI Fluor 10X, NA=0.5). The objective path was then passed through a dichroic filter (Semrock FF01–520/35–25) and then frame-projected through a 165mm focal length tube lens (Zeiss) onto the CMOS camera (Photometrics Prime 95B). The excitation arm consists of a 473 nm laser (OptoEngine, BL-II-473/1~100mW) coupled to a collimator (Thorlabs, F230FC-A). A 50 mm lens was then used to focus the beam through an dichroic mirror (Semrock, FF495-Di02–25×36) onto the back focal plane of the objective. The path was manually aligned to ensure uniform illumination of the three fiber bundles. The laser power was adjusted so that 0.25 mW was emitted, measured at the end of each fiber tip, in order to minimize photobleaching over multiple extended recording sessions.
Toader A.C., Regalado J.M., Li Y.R., Terceros A., Yadav N., Kumar S., Satow S., Hollunder F., Bonito-Oliva A, & Rajasethupathy P. (2023). Anteromedial Thalamus Gates the Selection & Stabilization of Long-Term Memories. bioRxiv.
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2

Multi-fiber Imaging System Setup

Check if the same lab product or an alternative is used in the 5 most similar protocols
A bundle of three imaging fibers with individual diameters of 0.6mm and core-to-core distance of 3.3 μm (Myriad Fibers, FIGH-30-650s) was coupled to the imaging objective (Nikon, CFI Fluor 10X, NA=0.5). The objective path was then passed through a dichroic filter (Semrock FF01-520/35-25) and then frame-projected through a 165mm focal length tube lens (Zeiss) onto the CMOS camera (Photometrics Prime 95B). The excitation arm consists of a 473 nm laser (OptoEngine, BL-II-473/1~100mW) coupled to a collimator (Thorlabs, F230FC-A). A 50 mm lens was then used to focus the beam through an dichroic mirror (Semrock, FF495-Di02-25×36) onto the back focal plane of the objective. The path was manually aligned to ensure uniform illumination of the three fiber bundles. The laser power was adjusted so that 0.25 mW was emitted, measured at the end of each fiber tip, in order to minimize photobleaching over multiple extended recording sessions.
Toader A.C., Regalado J.M., Li Y.R., Terceros A., Yadav N., Kumar S., Satow S., Hollunder F., Bonito-Oliva A, & Rajasethupathy P. (2023). Anteromedial Thalamus Gates the Selection and Stabilization of Long-Term Memories. Cell, 186(7), 1369-1381.e17.
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