Tnf α and

Manufactured by Thermo Fisher Scientific

The TNF-α assay is a laboratory tool used to measure the concentration of tumor necrosis factor alpha (TNF-α) in biological samples. TNF-α is a cytokine involved in various immune and inflammatory processes. The assay provides quantitative data on TNF-α levels, which can be utilized for research purposes.

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Lab products found in correlation

Ouabain, by MedChemExpress (1 mentions) U73122, by Bio-Techne (1 mentions) Carbachol, by Merck Group (1 mentions) Capsaicin, by MedChemExpress (1 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Hc067047, by MedChemExpress (1 mentions) Trpv4, by Abcam (1 mentions) U73343, by MedChemExpress (1 mentions) Mpo assay kit, by Abcam (1 mentions) Sb 705498, by MedChemExpress (1 mentions) Gsk1016790a, by MedChemExpress (1 mentions) Rn1734, by MedChemExpress (1 mentions) Glucose, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) Anti gapdh, by Proteintech (1 mentions) Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Interferon gamma ifn γ, by R&D Systems (1 mentions)

2 protocols using tnf α and

TRPV4-Mediated Inflammation Regulation

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All salts were supplied by Sangon Biotech. Glucose, caffeine, carbachol, and indomethacin were purchased from Sigma-Aldrich. Capsaicin, ouabain, SB705498, RN1734, RN1747, U73343, HC067047, GSK1016790A, methyl arachidonyl fluorophosphonate, AA, miconazole, and nystatin were purchased from MedChemExpress (MCE; Monmouth Junction). DSS was from MP Biomedicals (MP Bio). U73122 was from Tocris Bioscience (Tocris). Amiloride hydrochloride was from Molecular Probes, Inc (Molecular Probes). TNF-α and Fura-2/AM (Cat# F1221, Invitrogen) was from Invitrogen. TRPV4 (Abcam Cat# ab39260, RRID: AB_1143677), alpha 1 Sodium Potassium ATPase (phospho Y10) (Abcam Cat# ab124677, RRID: AB_10974461), Alexa Fluor 488 labeled anti-rabbit (Abcam Cat# ab150077, RRID: AB_2630356) antibodies, and MPO Assay Kit were from Abcam. Anti-GAPDH (Proteintech Cat# 60004-1-Ig, RRID: AB_2107436) was from Proteintech.

Wan H., Chen X.Y., Zhang F., Chen J., Chu F., Sellers Z.M., Xu F, & Dong H. (2022). Capsaicin inhibits intestinal Cl- secretion and promotes Na+ absorption by blocking TRPV4 channels in healthy and colitic mice. The Journal of Biological Chemistry, 298(5), 101847.

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Cytokine and Chemokine Measurement

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To measure the cytokine and chemokine levels in the culture supernatant and peritoneal lavage fluid, IL‐6, TNF‐α, and IL‐12p40 ELISA kits (Invitrogen) and interferon‐gamma IFN‐γ, granzyme B, CCL2, and CCL5 ELISA kits (R&D Systems) were used as per the manufacturer's instructions.

Ho T.L., Lee J., Ahn S.Y., Lee D., Song W., Kang I, & Ko E. (2023). Immunostimulatory effects of marine algae extracts on in vitro antigen‐presenting cell activation and in vivo immune cell recruitment. Food Science & Nutrition, 11(10), 6560-6570.

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