The cells were collected and lysed to obtain protein lysate. The resulting proteins were then electrophoresed through a 10% SDS-PAGE gel and transferred onto a PVDF membrane. After blocking with 5% non-fat milk overnight at 4°C, the membrane was incubated with the primary antibody (anti-SRF, Abcam, ab53147; anti-αSMA, Abcam, ab7817; anti-SDF1, clone K15C, MABC184, Millipore, Merck KGaA, Darmstadt, Germany; or anti-GAPDH, Protein Tech, 60004-1, China) for 1 hr at room temperature. The membrane was then washed 3 times with PBST (PBS with 0.1% Tween-20). After washing, the membrane was incubated with the corresponding horseradish peroxidase-conjugated goat anti-mouse or rabbit IgG at room temperature for 1 hr. The signals were visualized using the Immobilon Western Chemiluminescent HRP Substrate Kit (Millipore, USA).
Ab7817
Manufactured by Merck Group
Sourced in Germany
Ab7817 is a laboratory equipment product manufactured by Merck Group. It serves as a precision measurement tool for scientific applications. The core function of Ab7817 is to provide accurate and reliable measurements within a laboratory setting. Further details about its intended use or applications are not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab53147, by Abcam (1 mentions)
Immobilon western chemiluminescent hrp substrate kit, by Merck Group (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Ab83354, by Abcam (1 mentions)
Alexa fluor 633, by Thermo Fisher Scientific (1 mentions)
Mab377, by Abcam (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
Aria 3, by BD (1 mentions)
2 protocols using ab7817
1
Western Blot Analysis of Key Proteins
2
Multicolor Flow Cytometry of Brain Vasculature
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The brain microvessels or microvessel-depleted brain were stained with APC-or PE-conjugated antibodies to CD31 (BD Biosciences, 551262, 553373), PDGFR-b (mouse, Abcam, AB69506), a-SMA (mouse, Abcam, AB7817), NeuN (mouse, Millipore, MAB 377) and ASM (rabbit, Abcam, AB83354), and further stained with Alexa Fluor 594 (mouse, Invitrogen, A21044), Alexa Fluor 488 (rabbit, Invitrogen, A11008) or Alexa Fluor 633 (rabbit, Invitrogen, A21071). Data were collected on AriaIII (BD Biosciences) and analyzed using FlowJo software. Data were collected from 4-5 mice per group and analyzed in a double-blind fashion. Randomization procedures are not applicable to these experiments. G-power software was used for sample size estimation. No data were excluded.
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