Pas stain kit mucin stain

Manufactured by Abcam

Sourced in United States

The PAS (Periodic Acid-Schiff) Stain Kit is a laboratory staining solution used to detect the presence of mucins, glycoproteins, and glycogen in tissue samples. The kit provides the necessary reagents to perform the PAS staining technique, which is commonly used in histopathological analysis.

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Lab products found in correlation

Sc50 camera, by Olympus (1 mentions) Bx60f microscope, by Olympus (1 mentions) Superfrost plus, by Thermo Fisher Scientific (1 mentions) Glycogen assay kit, by Abcam (1 mentions) Sc 16816, by Santa Cruz Biotechnology (1 mentions)

2 protocols using pas stain kit mucin stain

Histology of Right Lung Tissue

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Right lungs were fixed in Histofix (Histolab), and paraffin embedded. Sections (3 µm) were cut with a microtome and placed on glass slides (Superfrost Plus; Thermo Fisher Scientific). Deparaffinization was performed using serial baths of xylene and ethanol. Staining was completed using Mayer hematoxylin and 0.2% eosin (Histolab) or Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (Abcam). Imaging of the slides was performed using an Olympus BX60F microscope with an SC50 camera (Olympus, Tokyo, Japan).

Tanner L., Bergwik J., Bhongir R.K., Pan L., Dong C., Wallner O., Kalderén C., Helleday T., Boldogh I., Adner M, & Egesten A. (2022). Pharmacological OGG1 inhibition decreases murine allergic airway inflammation. Frontiers in Pharmacology, 13, 999180.

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Quantifying Hepatic Glycogen and FGF15/FGFR4

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We detected the hepatic glycogen content using the Glycogen Assay kit (ab65620, Abcam, United States) according to manufacturer’s protocol. Sections of the paraffin-embedded tissues were prepared for PAS and immunohistochemistry staining. We stained the glycogen in hepatocytes with the PAS Stain Kit (Mucin Stain) (ab150680, Abcam, United States) according to the manufacturer’s protocol. And we also examined the ileal expression of FGF15 and hepatic expression of FGFR4 by immunohistochemistry staining. The antibody for FGF15 (sc-16816) and FGFR4 (sc-136988) were both purchased from Santa Cruz Biotechnology (United States). Percentage of PAS- positive cells was calculated and quantification of immunoreactive signal was performed using Image J software.

Wei M., Cao W.B., Zhao R.D., Sun D.P., Liang Y.Z., Huang Y.D., Cheng Z.W., Ouyang J., Yang W.S, & Yu W.B. (2023). Fibroblast growth factor 15, induced by elevated bile acids, mediates the improvement of hepatic glucose metabolism after sleeve gastrectomy. World Journal of Gastroenterology, 29(21), 3280-3291.

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