Nunclon delta surface sterile microtiter plates

Nunclon Delta Surface sterile microtiter plates (including the Edge 2.0 plate) and fetal calf serum were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Human serum from male AB plasma (sterile and filtered), antibiotics, antimycotic solution, methylthiazol terazolium salt and all other reagents were obtained from Sigma (St. Louis, MO, USA). All cell culture media and plasticware were procured from Whitehead Scientific (Cape Town, South Africa).
Clinical isolates of CRE, MRSA and VRE were obtained from Lancet Laboratories, Durban, South Africa, with approval BE394/15 from the Biomedical Research Ethical Committee of the University of KwaZulu-Natal. Five reference strains of bacteria, namely Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecium ATCC 35667, Staphylococcus aureus ATCC 29213 and Bacillus subtilis ATCC 6051 were supplied by the American Type Culture Collection (ATCC).

All the derivatives were dissolved in 5% DMSO. GIBCO RPMI-1640 cell culture media (with HEPES, L-glutamine and sodium pyruvate) was obtained from Life Technolgies (Carlsbad, CA, United States). Hyclone fetal bovine serum was purchased from GE Healthcare Life Sciences (Chicago, IL, United States). Phosphate Buffered Saline (PBS) was obtained from Lonza (Basel, Switzerland). Nunclon Delta Surface sterile microtiter plates (including the Edge 2.0 plate) were bought from Thermo Fisher Scientific (Waltham, MA, United States). Human serum from male AB plasma (sterile and filtered), antibiotics, antimycotic solution and all other reagents were obtained from Sigma (St. Louis, MO, United States).