Paraffin kidney sections (3 μm) were stained with periodic acid–Schiff (PAS) reagent by standard protocol. Immunohistochemical staining was performed with routine protocol [37 (link)]. Antibodies used were as follows: anti-caspase-3 (SC-65497; Santa Cruz Biotechnology), anti-KIM-1 (BA3537; Boster), anti-Sirtuin 6 (PB0375; Boster).
Anti sirtuin 6
Manufactured by Boster Bio
Anti-Sirtuin 6 is a laboratory reagent used to detect and quantify the expression of the sirtuin 6 protein in biological samples. It is a tool for research purposes.
Automatically generated - may contain errors
2 protocols using anti sirtuin 6
1
Immunohistochemical Staining of Kidney Sections
2
Immunoprecipitation and Western Blot Analysis
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IP experiments were performed with previous protocol [73 (link)]. Briefly, the cell lysates were immunoprecipitated overnight with anti-PGC-1α (SC-518038; Santa Cruz Biotechnology) and protein A/G plus agarose (sc-2003; Santa Cruz Biotechnology) at 4 °C. The precipitated complexes were washed three times with lysis buffer and boiled for 10 min in SDS sample buffer. And the next progression is similar to western blot. The primary antibodies of following western blot were as follows: anti-Sirtuin 6 (PB0375; Boster), anti-acetyl-lysine (9441 s; Cell Signaling Technology).
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