Unless otherwise specified, all reagents were from Sigma-Aldrich (Milan, Italy). Tissue culture media, antibiotics, and fetal bovine serum (FBS) were obtained from Life Technologies (San Giuliano Milanese, Italy). LPS (Ultra-Pure LPS-EB from Escherichia coli, 0111:B4 strain) was purchased from InvivoGen (InvivoGen Europe, Toulouse, France). Primary antibodies included: rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1, 019-19741, Wako Pure Chemical Industries, Ltd., Osaka, Japan), mouse anti-p65 (NF-κB p65, sc-8008, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-NOS2 (sc-7271, Santa Cruz Biotechnology). Alexa Fluor 488 and 555 secondary antibodies were from Invitrogen (A11008 and A21422, Milan, Italy). Enzyme-linked immunosorbent assay (ELISA) kits were obtained from Antigenix America (Huntington Station, NY, USA). Falcon tissue culture plasticwares were purchased from BD Biosciences (SACCO srl, Cadorago (CO), Italy).
Alexa fluor 488 and 555 secondary antibodies
Manufactured by Thermo Fisher Scientific
Sourced in Italy
Alexa Fluor 488 and 555 secondary antibodies are fluorescently-labeled antibodies used to detect and visualize primary antibodies in various biological applications, such as immunofluorescence, flow cytometry, and Western blotting. The Alexa Fluor 488 secondary antibody emits green fluorescence, while the Alexa Fluor 555 secondary antibody emits red fluorescence when excited by the appropriate light source. These secondary antibodies provide a sensitive and specific detection system for target proteins or cellular structures.
Automatically generated - may contain errors
2 protocols using alexa fluor 488 and 555 secondary antibodies
1
Microglia Activation Assay Protocol
2
Spirulina Modulates Inflammatory Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unless otherwise specified, all reagents were from Sigma-Aldrich (Milan, Italy). Tissue culture media, antibiotics, and fetal bovine serum (FBS) were obtained from Life Technologies (San Giuliano Milanese, Italy). LPS (Ultra-Pure LPS-EB from Escherichia coli, 0111:B4 strain) was purchased from InvivoGen (InvivoGen Europe, Toulouse, France). Dried Spirulina was purchased from a local health-food store. Primary antibodies included: mouse anti-p65 (NF-κB p65, Santa Cruz Biotechnology, Santa Cruz, CA, United States, Cat. sc-8008), mouse anti-iNOS (NOS2, Santa Cruz Biotechnology, Santa Cruz, CA, United States, Cat. sc-7271), and rabbit anti-Nrf2 (GeneTex Inc., Irvine, CA, United States, Cat. GTX103322). Alexa Fluor 488 and 555 secondary antibodies were from and Invitrogen (Milan, Italy, Cat. A11008 and A21422, respectively). Enzyme-linked immunosorbent assay (ELISA) kits were obtained from Antigenix America (Huntington Station, NY, United States). Falcon tissue culture plasticwares were purchased from BD Biosciences (SACCO srl, Cadorago (CO), Italy).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!