Mice were first stereotactically injected with AAV1-hSyn-GCaMP6s into the ARC. An optic fibre (400 μm diameter core; numerical aperture 0.37; Thorlabs) with a metal ferrule was then implanted unilaterally over the ARC (AP −1.45 mm, DV −5.8 mm, ML 0.3 mm from Bregma). The fibre and a custom-made headpost were then glued to the skull.
Optic fibre
Manufactured by Thorlabs
Sourced in United States
Optical fiber is a cylindrical waveguide made of glass or plastic that can transmit light signals over long distances with minimal loss. It consists of a core surrounded by a cladding layer, which helps to confine the light within the core. Optical fiber is used in a variety of applications, such as telecommunications, data transmission, and medical imaging.
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Lab products found in correlation
4 protocols using optic fibre
1
In Vivo Calcium Imaging of ARC
2
Optogenetic Manipulation of Cerebellar Purkinje Cells
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After craniotomy and retraining, Pcp2-Cre/Ai27 mice underwent to two task sessions (consecutive days) during light stimulation. An optic fibre (diameter 400 µm, Thorlabs, Newton, NJ, USA) was placed in the middle of the craniotomy perpendicular to the cerebellar surface. Three conditions were randomly intermingled for both go or no-go trials: a control condition of unaltered go or no-go trials and two conditions where a pulse of blue LED light (λ = 470 nm, duration = 250 ms, P = 5 mW) was given. During the first session, the light pulse was delivered either at time 0 ms (together with the acoustic cue) or after 300 ms (when the pole reached the top position); during the second session the light turned on either at 0 ms or at 550 ms during the response window, when licking was generally already ongoing. After the session the craniotomy was rinsed with saline and closed with Kwik-Cast. Purkinje cells of these mice were recorded in a subsequent session one to three days later.
3
Targeted Viral Infusion and Optogenetic Manipulation in Mouse AON
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Mice were anaesthetised with isoflurane (4% induction, 2% maintenance) and administered ketoprofen (5 mg/kg) for pain management. Viral vectors were bilaterally infused into the medial aspect of the AON (10° angle toward the midline, A/P: +3.00, M/L: ±1.15, D/V: −3.90) at a volume of 0.2–0.3 µL. Infusions were conducted using pressure ejection at a rate of 0.1 µL/min through a cannula connected by 20 cm of Tygon tubing to a 10-µL Hamilton syringe (Hamilton, Reno, NV). All infusions were allotted a 20-min interval to limit the viral spread. Optic fibres (200 µm core diameter, 0.39 NA; Thorlabs, Newton, NJ, USA) threaded through 1.25 mm-wide zirconia ferrules (Thorlabs) were implanted immediately dorsal to the infusion site (D/V: −3.80).
4
Optical Fiber and Multi-Channel Electrode Implants
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Optic fibres (diameter 100 µm, 0.22 NA, Thorlabs) were glued to zirconia ferrules (Precision Fibre Products) and implanted in 9 mice above STN (bilaterally AP: −2, ML: +/− 1.6, DV: 4.22 mm and AP: −0.27, ML: 1.6, DV: 4.47 mm with an angle of 20°). Five further animals were implanted in mPFC and/or ST with multichannel arrays made of 45 µm formwar-insulated tungsten wire (California Fine Wire Company) using the following coordinates: mPFC (AP: 1.5 mm, ML: 0.3 mm, DV: 3.1 mm) and ST (AP: −1.9 mm, ML: 1.6 mm, DV: 4.6 mm). Ground and reference wires were connected to a screw placed in the skull above the cerebellum. Implants were secured with additional bone screws and dental acrylic.
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